Date: Fri, 14 Jul 2006 15:04:08 -0400
From: Natalie Kuldell <nkuldell@MIT.EDU>
To: Louise Rafty <lrafty@dna20.com>
Subject: RE: new gene synthesis HEM1-HAm

Hi Louise-
Thanks for your quick reply to the other email. One more request: can I
get you to add the following flanking sequences to the optimized sequence for
HEM1-HAm that you already sent me? (warning, though, this flanking region has
high A/T %...if it's a real problem let me know and I can try to trim them a
bit...)

Upstream of ATG add: 5' gtttcttcgaattcgcggccgcttctagagAATTTTATTATATAGTTTAAGGGATAATATTTTATTAATATTTTTTTTATTTATTTATTTAATTATATTATATATATAATATATATATAACAATAAATTT
and then the ATG of the gene I sent before.

Downstream of the stop add: 5'AATTATATATCTAAATGATTAATATATATATTATTAATAATT
AACAATAATTAATATATTATAATTTATATATATATATTTTATATTATTATtactagtagcggccgctgcaggaagaaac

Thanks very much for the help!
Natalie