% Amneet Gulati % Manalis Lab % June 22, 2009 %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % Bead Concentration Conversion %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % INSTRUCTIONS: Please enter the following parameters to calculate % (1) stock concentration % (2) volume of stock and water to add to achieve target concentration % *REQUIRED PARAMETERS* solid fraction, density of the material, diameter of bead % *OPTIONAL PARAMETERS* target concentration, target total volume clear all clc % input the Solid Fraction in solution - generally 1% or 10% massfrac = 0.01; % input the Material Density: Polystyrene = 1.05 g/cm^3; Silica = 1.8 % g/cm^3; Melamine = 1.57 g/cm^3 density = 1.05; %(in g/cm^3) % input Particle Size (diameter) diameter = 8.62; %(in microns) % input Target Concentration target_conc = 1*10^6; % ( in # of beads/ml) % input Target Volume target_vol = 10; % (in ml) vol_bead = (4*pi/3)*(diameter/2).^3*10^-18; % (in m^3) stock_conc = (massfrac ./ (density*10^6))./vol_bead; disp(['Stock concentration is ', num2str(stock_conc),' beads/ml']) bead_stock_vol = (target_conc./stock_conc) .* target_vol; water_vol = target_vol - bead_stock_vol; disp(['Add ', num2str(bead_stock_vol), 'ml bead stock in ', num2str(water_vol), 'ml water.'])