KEY
dm = Drosophila melanogaster (house fly)
bm = Bombyx mori (silk worm)
purplesu = Strongylocentrotus purpuratus (purple sea urchin)

EVWFGFHQ = Amino acid sequences used to design primers to initiate PCR on conserved DNA region between them

ARGONAUTE  1
CLUSTAL W (1.83) multiple sequence alignment

dm              MSTERELAPGGPAQLHPHTLPLTFPDLQMTSTVGIIGKVYESQWTPSPTRPQSPSQAQTS
bm              ------------------------------------------------------------
purplesu        --------------------------------------MYQPPPHPNLPYGAPPFPQAPI

dm              FDTLTSPPAPGSSVNPTAVTSPSAQNVAAGGATVAGAAATAAQVASALGATTGSVTPAIA
bm              -MYPVGQPPAGETSGGAVGVAGAVGAPAGTPGAAPVAPSAAPVGAPAPGVAGGAAGLPLV
purplesu        QRPQAGQPPRQQAIPGPAPIQQQTATTLQGQTTLPPITAGGPPGPPAPAAPP-------G
                    .. *.  .:   ..     .        : .  .: ..  ..* ...         

dm              TATPATQPDMPVFTCPRRPNLGREGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKV
bm              SPAAAAPPDLPVLTCPRRPNLGHEGRPIMLRANHFQISMPRGFVHHYDVNIQPDKCPRKV
purplesu        GPGGPQPPRSDLVALPSRPGFGEDGRPIVLRANHFQVKIPSIEIFHYDVTITPDKCPRRV
                 .  .  *   :.: * **.:*.:****:*******:.:*   :.***:.* ******:*

dm              NREIIETMVHAYSK-IFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRV
bm              NREIVETMVHCYNK-IFGALKPVFDGRNNLYTRDPLPIGNDRMELEVILPGEGKDRVFRV
purplesu        NRDVIDTLVNAYKARYFQNNLPVFDGRRNMYTKEQLPLENERVELEATLPGEGKDRVFKT
                **::::*:*:.*.   *    ******.*:**:: **: *:*:***. ********:*:.

dm              TIKWQAQVSLFNLEEALEGRTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSSPEGYYH
bm              TIKWVAQVSLFALEEALEGRTRQIPYDAILALDVVMRHLPSMMYTPVGRSFFSSPEGYYH
purplesu        QIRYVGKVSLSLLESALKGEVEHMPYDAIQALDVIMRHLPSMRYTPVGRSFFSPPEEYFH
                 *:: .:***  **.**:*...::***** ****:******* **********.** *:*

dm              PLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPL
bm              PLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDINDQRKPL
purplesu        PLGGGREVWFGFHQSVRPSMWKMMLNIDVSATAFYNAQSVIDFLCEVLDIQNISEQRRPL
                ******************* ***************:**.**:*:******::*.:**:**

dm              TDSQRVKFTKEIKGLKIEITHCGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAK
bm              TDSQRVKFTKEIKGLKIEITHCGTMKRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAK
purplesu        SDSQRVKFTKEIKGLKIEITHCGNMRRKYRVCNVTKRPAQTQTFPWQLENGQTVECTVAK
                :********************** *:*********:**** *:** **************

dm              YFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATAR
bm              YFLDKYKMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVPGQRCIKKLTDMQTSTMIKATAR
purplesu        YFKERHNRILQYPHLPCLQVGQEQRHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATAR
                ** :::.  *:************::************.**********************

dm              SAPDREREINNLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTG
bm              SAPDREREINNLVRRANFNTDSYVKEFGLTISNNMMEVRGRVLPPPKLQYGGRVSS-LGG
purplesu        SAPDREKEIKNLVHKANFNNDRYVRQFGLSISNDMVTIEGRVLPAPKIQYGGKQNK----
                ******:**:***::*:**.* **::***:***.*: :.*****.**:****: ..    

dm              QLASPNQGVWDMRGKQFFTGVEIRIWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPI
bm              QQALPNQGVWDMRGKQFFMGVEIRVWAIACFAPQRTVREDALKNFTQQLQKISNDAGMPI
purplesu        TQAIPAQGVWDMRGKQFHTGVEIRVWAIACFAPQHQCREEALRTFTAQLQKISNDAGMPI
                  * * ***********. *****:*********:  **:**:.** *************

dm              IGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQ
bm              IGQPCFCKYATGPDQVEPMFKYLKSTFVQLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQ
purplesu        MGQPCFCKYAVGADQVEPMFRHLKSTYKGLQLIVVVLPGKTPVYAEVKRVGDTLLGIATQ
                :*********.*.*******::** *:  ***:********************:**:***

dm              CVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGD
bm              CVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPSLRPKVFNEPVIFLGVDVTHPPAGD
purplesu        CVQVKNVNRTTAQTLSNLCLKINVKLGGINNILVPNIRPRVFAEPVIFLGADVTHPPAGD
                ***.****:*:.******************.****.:**:** *******.*********

dm              NKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLIMFYKSTGGYKPHRI
bm              NKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIVHEMSSMVQELLIMFYKSTGGFKPHRI
purplesu        DKKPSIAAVVGSMDGHPSRYCASVRVQNHRVEIIQDLMGMVRELLMEFYRSTR-FKPARI
                :*************.*****.*:****:** **:::: .**:***: **:**  :** **

dm              ILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQS
bm              IMYRDGISEGQFLHVLQHELTAVREACIKLEAEYKPGITFIVVQKRHHTRLFCADKKEQS
purplesu        IMYRDGVSEGQFLQVLAQEMTAIRNACRSLEDFYEPGITFIVVQKRHHTRLFCAERREQI
                *:****:***** :** :*:**:*:** .**  *.*******************:::** 

dm              GKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTY
bm              GKSGNIPAGTTVDLGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFGSDELQCLTY
purplesu        GRSGNIPAGTTVDSGITHPLEFDFFLCSHAGIQGTSRPSHYHVLWDDNRFKADELQCLTY
                *:*********** ***** ****:**** ******************:* :********

dm              QLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHD-------SGEGSHQSGCSEDR
bm              QLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHD-------SGEGSHQSACSEDR
purplesu        QLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKDHDRYDHDSGGDGSSHHGASSYGR
                ************************************:**       .. .**:...* .*

dm              TPGAMARAITVHADTKKVMYFA
bm              TPGAMARAITVHAVTKKVMYFA
purplesu        SFEDMIRAVKVHDDTLKVMYFA
                :   * **:.**  * ******