II/X (part 1) (372-504)
tcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatt
<-------------------------------------------------------------II-------------------------------------------------------------------->
         |------------------X-Ptr-----------------------|                                                   |---X-RBS------||--X---->



*II/X/V
atttgagggggattcaatgaatatt....tcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaa
<---------------------------------------II-------------------------------------|
|---X-RBS------||------------------------X-------------------------------------|
                                 |------------------V-Ptr------------------------>
                                                                           |-----V-RBS----|
                                                                             |----------V---------->
*II/X/V
gcgttcgaaggcgactcgatgaatatt....tcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaACTAGTatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataa
<-----------------------------------------II-------------------------------------| SpeI |---X-RBS------||------------------------X---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
--|---X.1-RBS----|
                                 


II/X/V  (part 2) (774-851)
agatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaa
<--------------------------II----------------------------|
<--------------------------X-----------------------------|
            |------------------V-Ptr------------------------>
                                                     |-----V-RBS----|
                                                       |----------V---------->

**X.V juncture
agatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaAGATCTccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaa
<---------------------------X----------------------------||Bgl2|
                                                                |------------------V-Ptr-------------------------|
                                                                                                         |-----V-RBS----|
                                                                                                                         |---V--->


*V (1083-1116)
ctgcgcctcgttccggctaagtaacatggagcag
<---------V------------|
         |----VII-RBS---||--VII-->

**V.BglII.VII juncture 
ctgcgcctcgttccggctaagtaaGATATCgttccggctaagtaacatggagcag
<---------V------------|EcoRV|
                              |----VII-RBS---||--VII-->


VII (1092-1209)
gttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatga
|---VII-RBS----||--------------------------------------------VII-----------------------------------------------------|
                                                               |----------------VIII-Ptr---------------------|
                                                                                                  |--IX-RBS------||-IX->

VIII (1155-1314)
aatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctt
<--------------------VII------------------------------|
|----------------VIII-Ptr---------------------|                                                                                   |-----VIII-RBS-||---VIII---->
                                   |--IX-RBS------||----------------------------------------IX-------------------------------------------------------|                             
 


III (1491-1589)
tgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacGtgaaaaaatt
         |-----------------------III-Ptr----------------|               |-----III-RBS--||---III--->
<-------------VIII-------------|