Num	Seq_name	left_primer	right_primer	left_tm	right_tm	left_gc	right_gc	amplicon_size
1	>GRC13_0019_FC:7:1:16430:2355#GGCG	GTGAGACGAGAGACTTTGG	CTCTTCTTCTTCTTCCGCT	53.094	53.991	52.632	47.368	137
2	>GRC13_0019_FC:7:1:3166:19810#AACT	AGCTCGTGAAGCTGAAAC	ATCTGATGCACCATCTTTG	53.976	54.340	50.000	42.105	121
3	>GRC13_0019_FC:7:2:10609:6501#AACT	CTCATTGGCTGACACAGTC	ACCAATGATGGATGAGATG	54.952	53.857	52.632	42.105	158
4	>GRC13_0019_FC:7:2:15950:7942#AACT	GGAGACAAGAGTGAGACGAT	CTTCTTCTTCCTCTCCTTCA	54.216	53.805	50.000	45.000	191
5	>GRC13_0019_FC:7:3:5749:18097#CCCA	GGAAGAGCTTCTCGCTG	CATCATCTTCGTCCTCAAG	54.502	54.025	58.824	47.368	162
6	>GRC13_0019_FC:7:4:9633:2853#GTTG	AGTTTAGGGGTTTCAGGTG	TTTCATCAAGCAGTGAGATT	54.180	53.352	47.368	35.000	186
7	>GRC13_0019_FC:7:5:18876:19459#AACT	GATAAGGAAGTGGTCACCC	ACATGTCTTCCTCCCTTTC	54.294	53.975	52.632	47.368	151
7	>GRC13_0019_FC:7:6:3622:20269#AATC
8	>GRC13_0019_FC:7:7:18701:2551#AACT	TGTACCATCCTCACCAAAC	GAGGAGGAGGCACATAAAT	54.067	54.148	47.368	47.368	120
9	>GRC13_0019_FC:7:7:14435:3343#AACT	AAAGTCAGAGACTCCACGG	GAACTCACTGCTGGTGAAC	55.252	53.948	52.632	52.632	176
10	>GRC13_0019_FC:7:12:5232:7194#AACT	TCTTCTTCATCTTGGGGTT	GACCCAACAAGAATTCANN	54.624	53.335	42.105	41.176	180
11	>GRC13_0019_FC:7:12:3344:20980#AACT	GGTGATGATGACGACCA	TCATCATAATCATAGTCATCGTAG	53.931	53.766	52.941	33.333	170
12	>GRC13_0019_FC:7:14:4729:6179#AACT	TAGATCGTCATTGGAGCAT	TGCCACAATAAAATCACAA	54.003	53.378	42.105	31.579	136
13	>GRC13_0019_FC:7:14:15596:9260#CATC	TCCTTGTCTTCTGTTCCAA	CTGTAAATGCCAGCAGACT	54.085	53.922	42.105	47.368	146
14	>GRC13_0019_FC:7:18:5754:4349#TCCA	AAGAAACCAAACCCAAAAN	GGTTTCCTTTCGTGGTACT	54.252	54.164	33.333	47.368	116
15	>GRC13_0019_FC:7:19:6783:4740#AACT	ATGATGTTGATGATGACCG	CTTCTCCAATGGTTCAGTG	54.931	53.941	42.105	47.368	108
15	>GRC13_0019_FC:7:19:10782:5199#AACT
16	>GRC13_0019_FC:7:20:16551:3869#AACT	CAACTATCACCACACGACC	GTTCATGACGAGGAGGAG	54.644	53.736	52.632	55.556	178
17	>GRC13_0019_FC:7:20:13410:4549#GGTT	TGGTACAATTGTGCTTGTG	CTCACTGAACCCTAACCCT	53.780	54.131	42.105	52.632	144
18	>GRC13_0019_FC:7:20:6903:6353#AACT	ATGACAATGACCACGACA	TCATTCTTCATCATCATCG	53.596	52.521	44.444	36.842	134
19	>GRC13_0019_FC:7:20:8743:6778#CACC	TCTGGATATCTTTGAGCCA	GGCTGACCTTTACTTGTCN	54.162	54.017	42.105	50.000	164
20	>GRC13_0019_FC:7:20:14653:10798#AACT	CTAACTGCGTCTTTTCCAN	AACTTTGTTGTTGTTGCCA	53.578	55.089	44.444	36.842	121
20	>GRC13_0019_FC:7:20:9429:15621#AACT
21	>GRC13_0019_FC:7:20:2680:16794#AACT	ACGGAACTTACGCCTTTT	CTTAACTGCGGAGTTCTGA	54.959	54.080	44.444	47.368	155
22	>GRC13_0019_FC:7:21:2336:2206#AACT	GATGGCGATGACAATGA	CACCATCGTCGTGATCATA	54.300	56.211	47.059	47.368	110
22	>GRC13_0019_FC:7:25:9712:1969#AACT
23	>GRC13_0019_FC:7:25:18993:4579#CTGC	CTATCCTTGTTAGGCCCAC	AAAGGTCGGTGAAGAAGAG	54.731	54.444	52.632	47.368	119
24	>GRC13_0019_FC:7:25:10104:15451#AACT	CATCGTCATTGTCATCGT	GATGATGATGGTGAATGCT	53.242	54.030	44.444	42.105	142
25	>GRC13_0019_FC:7:26:3369:13467#AACT	AACACAGAGCCAATCACAT	CAAACATGGTCACCTTCAT	53.756	54.067	42.105	42.105	172
26	>GRC13_0019_FC:7:27:18895:16754#CAGC	ATCTCTACGGCCAAGATTC	TCGATCAGCCTCGATAATA	54.322	54.331	47.368	42.105	142
27	>GRC13_0019_FC:7:28:5258:18739#AACT	GGAGGAGAAGGAGGAGACT	ATAACACCATCTCCAGCAC	54.852	53.240	57.895	47.368	158
28	>GRC13_0019_FC:7:29:7736:13433#AACT	ATGCTTATGATGAGGAGGA	CATCATCATGGTTGTCATC	52.983	52.453	42.105	42.105	146
29	>GRC13_0019_FC:7:30:1303:3484#AACT	CATAAGGTGCCATTGAGAG	CTACAAATCCCGTTCCAG	54.121	53.927	47.368	50.000	139
30	>GRC13_0019_FC:7:31:9703:15602#ACCA	ACCACCACCACCATAAGAT	AGGAGCTTAATGGGAGAAA	54.987	54.080	47.368	42.105	122
31	>GRC13_0019_FC:7:33:16824:20670#AACT	TACCCAAACAACAACAACC	TCCCTAGGTTTCCCTTAGA	54.219	53.928	42.105	47.368	152
32	>GRC13_0019_FC:7:37:19773:12927#AACT	AGGAATCGATCCAAGTGAC	GTTTCAACCCTGCCTAAC	54.896	52.984	47.368	50.000	119
33	>GRC13_0019_FC:7:37:7002:13694#AACT	TCCTCATTATCGTAATAATCC	ACGATGATTATGATGGGC	51.174	54.013	33.333	44.444	120
33	>GRC13_0019_FC:7:39:4359:2531#AACT
34	>GRC13_0019_FC:7:40:11819:7078#AACT	TACCCATAGTGGCTGATTG	ACAACAACCCACTACTCCA	54.440	53.693	47.368	47.368	129
35	>GRC13_0019_FC:7:40:4239:19739#TCGG	GAGGAGGAGAGCTTTGAGT	CTCCTCATCATCTCCAACN	53.553	54.594	52.632	50.000	115
36	>GRC13_0019_FC:7:41:11027:1301#AACT	ATGACGTCTCAATAGCCAA	TGGCTAGCCTTTTCATAGA	54.103	53.725	42.105	42.105	156
37	>GRC13_0019_FC:7:41:4192:12493#AACT	AGGGGGAGTGAAAGAAATA	CACATCTCCCTCTCCCTAT	53.740	53.934	42.105	52.632	178
38	>GRC13_0019_FC:7:43:14843:11544#AACT	TGCCCGATAGATAGGTACA	CCAAGGTGTCTCTAGTGGA	54.114	53.458	47.368	52.632	152
39	>GRC13_0019_FC:7:44:7661:10702#AACT	CAACAACAAAAGAAATGGG	GATGCACAATCTGCTGTCT	54.052	54.570	36.842	47.368	153
40	>GRC13_0019_FC:7:44:4138:14726#AACT	GTCAGGGGAAGAGGAAATA	CCAGTCTTGACAAAATATGC	54.125	53.291	47.368	40.000	151
41	>GRC13_0019_FC:7:46:8775:19372#AACC	ATGATGGAGGTGGTGTTC	TAAAATGGCTACCGTCTCA	53.669	54.345	50.000	42.105	174
42	>GRC13_0019_FC:7:48:17001:11523#AACT	TGCACGTACTGTTGTTGAG	ACAAAATGTGCAACAACCT	54.017	53.922	47.368	36.842	146
43	>GRC13_0019_FC:7:48:4004:17887#AACT	TGATGATGAGGATGAGGAT	TAGGGTGACATGTTTCGTC	53.400	54.254	42.105	47.368	106
44	>GRC13_0019_FC:7:50:5088:4114#GGTG	TGATTGGAGAGAGGAGGA	CATCTGACCCATCTCCAC	53.798	54.340	50.000	55.556	153
45	>GRC13_0019_FC:7:50:3340:9292#GCAG	CCACCACAGATACTGCCTA	ATCCCTCCTATCATTCCAG	54.496	53.892	52.632	47.368	163
46	>GRC13_0019_FC:7:52:12480:15952#AACT	ACACGAAATTACAAGCGAG	TCGCTATTTTTGTTTCCAC	53.979	53.898	42.105	36.842	125
47	>GRC13_0019_FC:7:54:1866:6379#AACT	ACAATGTTTGTTGAGGTGG	TTCGTCATCATCAATGTCA	54.169	53.950	42.105	36.842	182
48	>GRC13_0019_FC:7:55:16834:3639#ATAA	CTCACCCTTTGATCTCCTT	GAGGATGACATAGCGACTG	54.172	54.049	47.368	52.632	143
49	>GRC13_0019_FC:7:56:12781:2448#AACT	GTTTGGATAGGCCAAAGTT	TGATGGTTAATGTCCACNN	54.286	53.748	42.105	41.176	110
50	>GRC13_0019_FC:7:57:13702:9554#AACT	AAAGAAGAAGAAGCTTGGG	AAAACTTGATGGCTCTCCT	53.874	53.934	42.105	42.105	151
51	>GRC13_0019_FC:7:58:18706:11691#CGTC	TCGCGAAACTTGTTCATAC	ACACTCTTTCAAACCCTCC	54.832	54.074	42.105	47.368	140
52	>GRC13_0019_FC:7:60:19069:7066#AACT	TGAGGAGCATGAAGAAGAG	CCTCCTTTTTGTCCTTGAT	53.879	54.220	47.368	42.105	146
53	>GRC13_0019_FC:7:61:3748:8409#GTTG	GTTTGAGAGAGGAGGAGGA	CACCTCAACCACCACTATG	54.264	54.660	52.632	52.632	159
54	>GRC13_0019_FC:7:61:13146:19764#CGGC	ATCTCTACGGCCAAGATTC	CTTAACAACAACCACCACC	54.322	53.759	47.368	47.368	131
55	>GRC13_0019_FC:7:61:3392:20864#AACT	TAATTGAATAAAACGGGGG	CGGCTCAAATCAATCATAC	55.119	54.057	36.842	42.105	164
56	>GRC13_0019_FC:7:62:7199:10851#AACT	CCAGTTCAGTGGACTTGAT	CTTCTTCATCGTCGTCATC	53.243	54.006	47.368	47.368	126
57	>GRC13_0019_FC:7:63:9074:5662#AACT	TGCCATGTGATATTTTGAA	TTCTATCACCACCACTACCA	53.270	53.869	31.579	45.000	151
58	>GRC13_0019_FC:7:63:15077:13644#AACT	ACCAGAAATCAATGAAGAAGA	CCATCAACATGATGACCA	53.988	54.032	33.333	44.444	161
59	>GRC13_0019_FC:7:64:10039:7239#AACT	AAGGAGGTAGTGGTTGCTT	TATCTTCCTCCTCCTCCAT	53.896	53.575	47.368	47.368	133
60	>GRC13_0019_FC:7:64:10477:8525#AACT	AGGCAGATCAATTAACCAA	TTGACTCTAACGCCAAAAG	53.177	54.131	36.842	42.105	138
60	>GRC13_0019_FC:7:64:17740:10129#AACT
61	>GRC13_0019_FC:7:65:13979:9108#AACT	GAACAACAACAACAGGGAT	GCTGGTACATTCTTTGGTG	53.116	54.021	42.105	47.368	125
62	>GRC13_0019_FC:7:66:7877:16287#AACT	CGAGTCGCTTCTCTACTTG	TTATGGAAGTTGCCTGGTA	53.800	54.170	52.632	42.105	154
63	>GRC13_0019_FC:7:67:16820:3892#AACT	AAAGCACAGTGATGAAACC	ATGTCCTTTTGAGCCTTTT	53.493	53.988	42.105	36.842	153
64	>GRC13_0019_FC:7:67:14277:6363#AACT	GAACTGCAGTCAAATGCTC	CCAAAATCAGTCTCAGTGG	54.279	53.941	47.368	47.368	187
65	>GRC13_0019_FC:7:67:9069:18251#AACT	AGAATCCTAGCCAGACCAC	AGGAGAGTGGGGGTTCTAT	54.195	55.047	52.632	52.632	169
66	>GRC13_0019_FC:7:67:9425:20974#AACT	GCTGTTTCTTCCTTGAACTC	AGCAACCATCATCATCATC	54.175	54.030	45.000	42.105	128
67	>GRC13_0019_FC:7:68:18572:8809#GCAA	TAAAACACACCCCAAGCTA	TGGGTTATTGTTGTGGTTC	54.265	54.121	42.105	42.105	145
68	>GRC13_0019_FC:7:71:12718:1554#AACT	TCATCTTCTACCAGCAGGA	AGTGACCATGATTGGACTG	54.212	54.010	47.368	47.368	153
69	>GRC13_0019_FC:7:71:14542:8735#AACT	TCCTCATCATCATTGTTT	TCATGATGAGTATGACTATGANN	48.783	52.379	33.333	33.333	104
70	>GRC13_0019_FC:7:71:15983:12531#AACT	ATCCGAAAAGTGGTTGATT	CCTAATAGCCAACAACGAA	54.509	53.954	36.842	42.105	163
71	>GRC13_0019_FC:7:72:12400:9804#AACT	ACGGAACTTACGCCTTTT	CTTAACTGCGGAGTTCTGA	54.959	54.080	44.444	47.368	155
72	>GRC13_0019_FC:7:72:9461:16910#CACC	TCTGGATATCTTTGAGCCA	TGATGATATGGCTGACCTT	54.162	53.817	42.105	42.105	173
73	>GRC13_0019_FC:7:73:12351:17241#CACG	TGTTGATCGTCACCTTCTT	AAGCTGAGGAAGATAAGCC	53.922	53.800	42.105	47.368	144
73	>GRC13_0019_FC:7:76:10430:12966#AACT
74	>GRC13_0019_FC:7:79:10798:15390#AACT	AGACCCATTAGCAACACAA	GAGGAGGAGGAGGATATTTT	54.021	53.970	42.105	45.000	194
75	>GRC13_0019_FC:7:80:13838:5803#AACT	AGCAACCATCATCATCATC	GTAGCCTCAATGTCCTTCTC	54.030	53.945	42.105	50.000	179
76	>GRC13_0019_FC:7:81:6251:14377#AACT	TACGCCAAGAAGAAGAAGA	CGGCATAGACCGAATTT	53.721	54.484	42.105	47.059	183
77	>GRC13_0019_FC:7:85:18859:4775#AACT	CTAAACCAGCATCAACCAC	GAAAAAGCCCCTAATTTTG	54.021	54.173	47.368	36.842	136
78	>GRC13_0019_FC:7:85:18197:11212#AACT	AATCATGAGAGAACAAGGCT	ATGAACTTTTCCTTGGCTT	53.945	53.988	40.000	36.842	148
79	>GRC13_0019_FC:7:85:16070:14165#AACT	CAAACCCAATACATTCCAG	GGTTAATGGAGGCTAGGTC	53.818	53.761	42.105	52.632	151
80	>GRC13_0019_FC:7:86:5996:6425#AACT	CGTTTTGGTCAAAGAACTC	GAGGAACGAGCAGTGAGTA	53.265	53.819	42.105	52.632	129
81	>GRC13_0019_FC:7:91:11979:13113#GGTA	AGAAGGGTAGTTTTGGGAA	AACCATATGCTCTTCTCCC	53.835	54.148	42.105	47.368	154
82	>GRC13_0019_FC:7:91:9721:15042#AACT	TATTTGGAGGAGTTGTTGG	TGTCCTTTACTCTCCTCCC	53.501	54.172	42.105	52.632	135
83	>GRC13_0019_FC:7:92:17819:7991#AACT	GATGGTAGTTACCGTCCGT	AACAACAAATCAATCGTGG	54.911	54.301	52.632	36.842	167
84	>GRC13_0019_FC:7:96:12412:5794#AACT	AAAGGGAAAATCAGAGAGG	ATCATCATCATCATCCTCG	53.434	53.942	42.105	42.105	126
85	>GRC13_0019_FC:7:96:12208:11162#AACT	CCCCAAATCCTAAAACAAT	GGGTCGTAGAGGTTGAACT	54.091	54.114	36.842	52.632	199
86	>GRC13_0019_FC:7:98:6323:14839#AACT	GTTTCAACCCTGCCTAAC	AGGAATCGATCCAAGTGAC	52.984	54.896	50.000	47.368	119
87	>GRC13_0019_FC:7:99:6991:12750#AACT	TTCTTTCCAAGTGCATAGC	CACCTTGACTGGAACCATA	54.017	54.271	42.105	47.368	113
88	>GRC13_0019_FC:7:99:2760:20846#AATG	CAGTTTATGCAGGAGGAGA	TTTTGTGTAGTGATGGCCT	53.797	54.021	47.368	42.105	165
89	>GRC13_0019_FC:7:100:18138:7434#AACT	GGTTGTCCTCTCCTGAGTT	CTTCTTGGTCAAGGGATGT	54.020	55.001	52.632	47.368	195
89	>GRC13_0019_FC:7:100:14930:17800#AACT
90	>GRC13_0019_FC:7:101:7891:13450#AACT	ACTACACATCACCACCACC	ACTGGCTTCACTGGTACTG	53.752	53.590	52.632	52.632	161
91	>GRC13_0019_FC:7:104:12814:19285#AACT	ACTCAAAGCTCTCCTCCTC	GAGTTACCAGAACTCGACG	53.553	53.227	52.632	52.632	117
92	>GRC13_0019_FC:7:105:12463:3265#AACT	CTATTGTGACTCCACCACC	ATGGGACATGTTTCTTGTG	53.585	54.067	52.632	42.105	113
93	>GRC13_0019_FC:7:108:6657:4006#CCCA	CTCTTTGCATTTGTGCTTT	CAACCTGCTGTCGTAAAAA	54.114	54.935	36.842	42.105	126
94	>GRC13_0019_FC:7:108:4432:17205#AACT	GACTCAGGAAAAACGGTCT	GGCATCATCATCATCAAGT	54.252	54.030	47.368	42.105	117
95	>GRC13_0019_FC:7:108:19023:18521#AACT	TCTTCCTCATTTGGTGAAA	AAAGGCTCATCAAAAGTTG	54.039	52.967	36.842	36.842	184
96	>GRC13_0019_FC:7:109:8230:9234#AACT	ACCTCAGAATACCACCACC	TGTTGTTGTTGTTGTTGTTG	54.669	53.771	52.632	35.000	178
97	>GRC13_0019_FC:7:109:11313:9554#AACT	ATCAGGGTAGCCTCCATAC	CTCCTCCTCCATATGATCC	54.012	54.296	52.632	52.632	129
98	>GRC13_0019_FC:7:109:6638:19532#AACT	TACCCAAACAACAACAACC	CATGGTTAGAGGGGTCTTC	54.219	54.487	42.105	52.632	169
99	>GRC13_0019_FC:7:110:13176:7559#AACT	CTTAACTGCGGAGTTCTGA	ACGGAACTTACGCCTTTT	54.080	54.959	47.368	44.444	155
100	>GRC13_0019_FC:7:111:12525:1997#AATC	CCTCTCAGCGAATAAAGAA	TGATGATGCTGATGTTGAC	53.240	53.412	42.105	42.105	161
101	>GRC13_0019_FC:7:112:17529:2552#AACT	AACAGGGTAGCCTCCATAC	CTCCTCCTCCATATGATCC	54.108	54.296	52.632	52.632	129
102	>GRC13_0019_FC:7:112:9038:9209#AACT	TGATGATGATGATGAACAAN	TCATCATTTTAGTGGTCATTG	52.339	53.591	31.579	33.333	138
103	>GRC13_0019_FC:7:113:12678:8810#AACT	ATCCTTGTGCTTTTCCTTT	CGTCTTCCTCTTTCTCCTC	53.988	54.034	36.842	52.632	153
103	>GRC13_0019_FC:7:114:3252:4542#AACT
104	>GRC13_0019_FC:7:114:6139:6187#CTTC	TCCCCACTCTCATCATTTA	CGTTCTCTGGTTTGAAAAA	53.794	53.916	42.105	36.842	138
104	>GRC13_0019_FC:7:114:1425:15132#AACT
105	>GRC13_0019_FC:7:117:12589:2083#AACT	CCAACAGTTATTTGGCAAT	TGAGCAAGATCAACAACAA	53.591	53.477	36.842	36.842	172
106	>GRC13_0019_FC:7:117:7496:8651#CTAC	ACTACCCACAACAGCAACA	CTACGATGTATGGGATTCG	54.926	53.937	47.368	47.368	177
107	>GRC13_0019_FC:7:120:7676:10003#AACT	AAAGATCCATCAGAGGAGG	TGAAAAGCGCATAATACNN	54.074	53.818	47.368	35.294	116
108	>GRC13_0019_FC:7:120:13344:11763#AACT	TCCTCCTCCAACTCTTCTC	ATTGAGGGAGGACGGAG	54.264	55.837	52.632	58.824	174