LOCUS       M13mp18                 7249 bp    DNA     circular     19-OCT-2007
DEFINITION  Cloning vector M13mp18, complete sequence.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Cloning vector M13mp18
  ORGANISM  Cloning vector M13mp18
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Stewart,F.J.
  TITLE     Sequence of M13mp18 and M13mp19
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Slatko,B.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     Corrects errors in M13mp18 GenBank entry X02513 and NEB sequence
            file dated 18 Apr 1994 based on 2002 sequencing of complete M13mp18
            phage genome at NEB (Stewart, F.J., unpublished).
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Cloning vector M13mp18"
                     /mol_type="other DNA"
     gene            join(6848..7249,1..831)
                     /gene="II"
     CDS             join(6848..7249,1..831)
                     /gene="II"
                     /note="replication"
                     /codon_start=1
                     /product="pII"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSA
                     RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA"
     gene            496..831
                     /gene="X"
     CDS             496..831
                     /gene="X"
                     /note="replication"
                     /codon_start=1
                     /product="pX"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
     gene            843..1106
                     /gene="V"
     CDS             843..1106
                     /gene="V"
                     /note="replication"
                     /codon_start=1
                     /product="pV"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     gene            1108..1209
                     /gene="VII"
     CDS             1108..1209
                     /gene="VII"
                     /note="minor coat protein"
                     /codon_start=1
                     /product="pVII"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     gene            1206..1304
                     /gene="IX"
     CDS             1206..1304
                     /gene="IX"
                     /note="minor coat protein"
                     /codon_start=1
                     /product="pIX"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     gene            1301..1522
                     /gene="VIII"
     CDS             1301..1522
                     /gene="VIII"
                     /note="major coat protein"
                     /codon_start=1
                     /product="pVIII"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY
                     IGYAWAMVVVIVGATIGIKLFKKFTSKAS"
     gene            1578..2852
                     /gene="III"
     CDS             1578..2852
                     /gene="III"
                     /exception="alternative start codon"
                     /note="minor coat protein"
                     /codon_start=1
                     /transl_table=11
                     /product="pIII"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKT
                     LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG
                     GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR
                     NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFV
                     CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF
                     DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT
                     GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKI
                     NLFRGVFAFLLYVATFMYVFSTFANILRNKES"
     gene            2855..3193
                     /gene="VI"
     CDS             2855..3193
                     /gene="VI"
                     /note="minor coat protein"
                     /codon_start=1
                     /product="pVI"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK"
     gene            3195..4241
                     /gene="I"
     CDS             3195..4241
                     /gene="I"
                     /note="phage assembly"
                     /codon_start=1
                     /product="pI"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV
                     SIKKGNSNEIVKCN"
     gene            3915..4241
                     /gene="XI"
                     /note="also called I*"
     CDS             3915..4241
                     /gene="XI"
                     /note="phage assembly"
                     /codon_start=1
                     /product="pXI"
                     /translation="MKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVVSQT
                     YDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGNSN
                     EIVKCN"
     gene            4219..5499
                     /gene="IV"
     CDS             4219..5499
                     /gene="IV"
                     /note="phage assembly"
                     /codon_start=1
                     /product="pIV"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYI
                     DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL"
     rep_origin      5487..5867
                     /note="M13 origin of replication (- +)"
     gene            6216..6722
                     /gene="lacZalpha"
     CDS             6216..6722
                     /gene="lacZalpha"
                     /codon_start=1
                     /product="beta-galactosidase alpha fragment"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADT
                     VVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRI
                     IFDGVPIG"
     misc_feature    6230..6286
                     /gene="lacZalpha"
                     /note="multiple cloning site (EcoRI-HindIII)"
BASE COUNT     1766 a   1530 c   1534 g   2419 t
ORIGIN
        1 aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttata tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag cattatattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattcttttg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct
     1621 attctcactc cgctgaaact gttgaaagtt gtttagcaaa atcccataca gaaaattcat
     1681 ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggctgtc
     1741 tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat
     1801 gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt
     1861 ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta
     1921 ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa
     1981 accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc
     2041 agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc
     2101 aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt
     2161 atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg
     2221 atttatttgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg
     2281 ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg
     2341 gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg
     2401 attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg
     2461 aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg
     2521 ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg
     2581 gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt
     2641 taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt
     2701 ttgtctttgg cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat
     2761 tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt
     2821 ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt
     2881 attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct
     2941 taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg
     3001 gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt
     3061 tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct
     3121 ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga
     3181 ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc
     3241 tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc
     3301 ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc
     3361 ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt
     3421 cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata
     3481 cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta
     3541 aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc
     3601 gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt
     3661 ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg
     3721 ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata
     3781 ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt
     3841 ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa
     3901 atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt
     3961 gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg
     4021 aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc
     4081 agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata
     4141 gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca
     4201 ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt
     4261 gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt
     4321 gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt
     4381 actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct
     4441 gttttacgtg caaataattt tgatatggta ggttctaacc cttccattat tcagaagtat
     4501 aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat
     4561 gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact
     4621 tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag
     4681 tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt
     4741 agtgctccta aagatatttt agataacctt cctcaattcc tttcaactgt tgatttgcca
     4801 actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat
     4861 ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc
     4921 ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta
     4981 gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt
     5041 attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt
     5101 actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt
     5161 caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt
     5221 ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt
     5281 actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc
     5341 ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa
     5401 atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta
     5461 tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5701 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5821 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
     5881 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     5941 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     6001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     6241 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
     6301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6421 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
     6481 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
     6541 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
     6601 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
     6661 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt cctattggtt
     6721 aaaaaatgag ctgatttaac aaaaatttaa tgcgaatttt aacaaaatat taacgtttac
     6781 aatttaaata tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg
     6841 ggtacatatg attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc
     6901 cagactctca ggcaatgacc tgatagcctt tgtagatctc tcaaaaatag ctaccctctc
     6961 cggcattaat ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc
     7021 cggcctttct cacccttttg aatctttacc tacacattac tcaggcattg catttaaaat
     7081 atatgagggt tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt
     7141 attacagggt cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt
     7201 gcttaatttt gctaattctt tgccttgcct gtatgattta ttggatgtt
//
