LOCUS        PCRp_GFPst_Insert         846 bp ds-DNA   linear       27-FEB-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    15..731
                     /label=GFPuv
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    732..776
                     /label=Linker
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    777..797
                     /label=Sortase Tag
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=green
     misc_feature    804..821
                     /label=HISx6
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=green
ORIGIN
        1 acgtacgttc tagaATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG
       61 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG
      121 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC
      181 CATGGCCAAC ACTTGTCACT ACTTTCTCTT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG
      241 ATCATATGAA ACGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC
      301 GCACTATATC TTTCAAAGAT GACGGGAACT ACAAGACGCG TGCTGAAGTC AAGTTTGAAG
      361 GTGATACCCT TGTTAATCGT ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA
      421 TTCTCGGACA CAAACTCGAG TACAACTATA ACTCACACAA TGTATACATC ACGGCAGACA
      481 AACAAAAGAA TGGAATCAAA GCTAACTTCA AAATTCGCCA CAACATTGAA GATGGATCCG
      541 TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC
      601 CAGACAACCA TTACCTGTCG ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGCGTG
      661 ACCACATGGT CCTTCTTGAG TTTGTAACTG CTGCTGGGAT TACACATGGC ATGGATGAGC
      721 TCTACAAATA AGGTGGCGGT GGCTCTGGCG GCGGTGGTTC TGGTGGTGGC GGTTCCCTGC
      781 CGGAAACCGG CGGTGGCCCG CGGCACCATC ACCACCATCA TCCCGGGTAA cctgcaggac
      841 gtacgt     
//