LOCUS pDONR-Express 7385 bp DNA circular 25-SEP-2011 SOURCE ORGANISM COMMENT VNTNAME|pDONR-Express| COMMENT VNTAUTHORNAME|Mohammad Dezfulian| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers promoter 76..162 /vntifkey="30" /label=EML\Promoter misc_feature 163..391 /vntifkey="21" /label=attP1 misc_marker 2166..2462 /vntifkey="22" /label=ccdB misc_feature 1440..2099 /vntifkey="21" /label=CmR misc_feature complement(2346..2558) /vntifkey="21" /label=attP2 misc_marker 2598..3392 /vntifkey="22" /label=KanamycinR misc_marker 3535..4545 /vntifkey="22" /label=SpecR misc_feature 5379..6470 /vntifkey="21" /label=Lac\I\ORF rep_origin 6661..7334 /vntifkey="33" /label=PUC\origin BASE COUNT 1776 a 1900 c 1911 g 1798 t ORIGIN 1 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 61 ggaagcggaa gagcgtgttg acaattaatc atcggcatag tatatcggca tagtataata 121 cgaggaattg tgagcggata acaattccca aggtgaggaa ctaaataatg attttatttt 181 gcctgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa 241 ctttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 301 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 361 gaatcaacta cttagatggt attagtgacc tgtagtcgac cgacagcctt ccaaatgttc 421 ttcgggtgat gctgccaact tagtcgaccg acagccttcc aaatgttctt ctcaaacgga 481 atcgtcgtat ccagcctact cgctattgtc ctcaatgccg tattaaatca taaaaagaaa 541 taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc tacctattca 601 tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa gaacaatttc acaactctta 661 tacttttctc ttacaagtcg ttcggcttca tctggatttt cagcctctat acttactaaa 721 cgtgataaag tttctgtaat ttctactgta tcgacctgca gactggctgt gtataaggga 781 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg 841 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt 901 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac 961 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc 1021 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta 1081 aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa 1141 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca 1201 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata 1261 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc 1321 atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa 1381 tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc acgcggcgtt 1441 tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 1501 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 1561 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 1621 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 1681 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 1741 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 1801 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 1861 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa 1921 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 1981 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 2041 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 2101 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 2161 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa 2221 agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt 2281 gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttag 2341 tcgactacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg 2401 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta 2461 tatcatttta cgtttctcgt tcagctttct tgtacaaagt tggcattata agaaagcatt 2521 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc 2581 atccagctga tatcgcctca attgaacaag atggattgca cgcaggttct ccggccgctt 2641 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2701 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 2761 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 2821 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 2881 gtgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 2941 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3001 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3061 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3121 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 3181 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3241 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3301 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3361 ccttctatcg ccttcttgac gagttcttct gagctctaga ccagccagga cagaaatgcc 3421 tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca 3481 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc 3541 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt 3601 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag 3661 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag 3721 cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta 3781 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 3841 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 3901 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 3961 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 4021 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 4081 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 4141 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 4201 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 4261 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 4321 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 4381 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag 4441 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 4501 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc gaccgagatg 4561 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt 4621 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg 4681 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac 4741 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc 4801 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta 4861 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc 4921 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa 4981 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg 5041 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga 5101 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg 5161 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc 5221 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg 5281 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg 5341 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg 5401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 5461 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc 5521 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg 5581 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg 5641 tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc 5701 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca 5761 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc 5821 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc 5881 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc 5941 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt 6001 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca 6061 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga 6121 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc 6181 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg 6241 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt 6301 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc 6361 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca 6421 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga 6481 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc 6541 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt 6601 gaggccgttg agcaccgccg ccgcaaggaa tggtgcgcgt cgttccactg agcgtcagac 6661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 6841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 7021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 7081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 7141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 7201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 7261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 7321 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7381 gcctt //