LOCUS NEWMOL 3062 bp DNA circular 29-JUN-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|414503545| COMMENT VNTDBDATE|414503545| COMMENT VNTNAME|NEWMOL| COMMENT VNTAUTHORNAME|Alain Viel| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT #216=(CMutObj (CStringList "g" "a" "2843") (CStringList "EcoRI" "1")) COMMENT #217=(CMutObj (CStringList "c" "t" "2915") (CStringList "EcoRI" "1")) COMMENT #218=(CMutObj (CStringList "t" "a" "2981") (CStringList "EcoRI" "1"))) 1 COMMENT (CStringList "EcoRI" "PstI") 273 272 (CObList))) FEATURES Location/Qualifiers primer 589..613 /vntifkey="27" /label=mutagenesis_601 BASE COUNT 801 a 750 c 766 g 745 t ORIGIN 1 tacagaatcc agcacaacgg ctttatcgcg aggccttagc tttctcggtg ctcgggttga 61 cgactgagct gagacaagaa tacctaagcg cgatcgctgg ctagctctcc ggcccttttt 121 cttgaacgcc gcgcacaatt cgcgagagtt cgcttttctc atcgacggta atccgtgttg 181 gcgaacctga aataatccgc tcaaagttcc ggaaagaatc cttgatgtcc ggccctttgt 241 cgctgatcat gaattcgcgg attgctttgt catgccaaga tccgcgcatc ttgaagacgt 301 taatggcgcg ggacatttcg ccacgaatct cgacgtattg gagcaagata atcgtatccg 361 taattgttga gatatgggag tcagtaatcg aatgcgctcc cataaattga tcactggtat 421 ttgtgaatag tcccgtgatt tcttcttgtt tcgcgtagcc agtgacacca attacaaatt 481 ggcggaaggc attgttgcta acgccccgcg ccaaagcaga gagggagtcg attgcaatac 541 gagctggctt aaagtcattg atctccgact taataatctg caaatggtct tcaagacctg 601 cagattcagg ataggcgcaa acaattttga ggaggttttg gcgctccatc tcctcaaagt 661 ccattcccca tgaataggca ttgcggagca gctgagctcg tgactcttca taagcgaaca 721 gaatcgcccg ctctttgtta gcacaagcat tctcaacgaa acggctaact aacagagttt 781 taccagtgcc tgtagcgcca gttgccagaa tgattgagtc cttaaagaag cccccaccac 841 acatttcatc gagtcggacg acaccagatg aaacacgcac gttcgacgat cgctgcgtaa 901 ggcgcattgc cccgagcggg aagatattga tgccatgatc cgtaatcgtg aacggatatt 961 cccctttcat gtggctggtg ccacgtagct tgaggatttc gagggtgcgg cgacggcgct 1021 ccccttccaa aacgttgcgg agaatcacga cgttatcgga gacaaattcc tcaacaccgt 1081 aacgagcgat cgggccatat tcctcgatac gctcggtggt catgaccgta gttgccccaa 1141 tttgttttag gcgagctacc aaccgaaaga gttcgcggcg aaccacagaa gaggcatcat 1201 attgctggaa aacggacgtg accgagtcaa ttgaaacccg ccgcgctcga tacttttgaa 1261 ttgcataatt aatccgctca atcagagcag agagatcgaa gccgccaaca acctcttgac 1321 cttctggatc gggtgaagca tcaagaataa atagtttgcc ctcatcgacc agcttggcta 1381 aatcccagcc aaaactacgg gcgtttttaa tgatatcttg cggggtttct tcgaaagtaa 1441 cgaaaacccc aggctcatca aactcgataa taccgttata gagaaattga atagaaaaaa 1501 gggtcttgcc ggttcctgaa gtaccactaa cgagggtcga tcgcccgatt ggaagaccgc 1561 catgactaat atcatcaaag ccttcaatca tcgtgcgcat cttagcgatg gcttggtgct 1621 cagaattatt attagggcta gtcatctcag cggaagtcat cttaaaagag ggtgaagtca 1681 ggtagtacca acaaagcagt taacgacagt tagaagtcgt cggaatcttg aagttcgccg 1741 tagagtaaat caaggccaat caaaactttc tcacggtcgg ataaatcacc aataatccgt 1801 cggacaggca gtggtagaac cttggcgagg gttggcgtcg ctaggatttt atcctcttct 1861 gccaactgag gatttttgag aacatcgatc acctttagag cataaacacc ttgaaattca 1921 acttcgagaa tgttcttgag cgttttgagg gcacggactg agtttggagt attgccggcg 1981 acgtagagct tgagaatgta ggttttacga gggctcatac gcagatcaac ggggtagcaa 2041 cagcaatcaa accatctaac aggataaaga gagaaattga gccgagctta agacctcctt 2101 taccttttca ggtttctcgt gggatagacc gtcgatacat ctcacaaaga tgtgcaatta 2161 catcaattaa agtcagccga taatccagca aaatgtcctc tgaacgtccc tctacgcgga 2221 gcttcttggc aaactcgtcc atcagctcca tgtgaatttc taccactttg gtgactggaa 2281 catcggcaaa gaaagccatg ttgacgaagt tgtcaatgct ctggttgagg ttgctattcg 2341 gcgaaaaata gctcaaaacg atttcacgat agctagtctg catcgcttgg tgcagctttt 2401 ggctttcgta ggcgggtagg ttgcgcagaa agcgatcggg atcacgcttg tagtagaccc 2461 cgagatagcc taggcgctct tgtagtcgct gagcgaggtc ccgctgctgg ctcgatagct 2521 cgggatcgtg gttggccccc atcagcatga tgtggtcggc catggtctcg accggggcta 2581 agcgcagaaa ttcagccagt gcagcatcaa cttggtaggg caattgctcg agctgatgga 2641 taccgaggtg cagttcagcg ctgtgataga gctgttcttt ggctggttca tcgggatcct 2701 cactgtcgcg atcgcctacg acaatcgctg gtaccaccac tccctcaaag cagagctgct 2761 gaacaactgc cctgaagctg ggattggctg ccactaaaat caggcagtcg atttggtcac 2821 gatgggtttg ggcatactcc aagagcattt cgccagactc acagacttgg agttgatagc 2881 gatcggccga cagcgcccgc tggcaatcct gcaaaattgc cgtcgattcc acccaaatgc 2941 agattgcaat ttgcgagagc acgcagtcgc tcctgtcagg actgagtctg cagacagatt 3001 aatctctgga caaaggaaaa atttaattta gcctagcact gcagctcatg aggccgcgat 3061 cg //