Sample Count Frequency CD69_MFI CD69_CV CD69_MAD CD203_MFI CD203_CV CD203_MAD CD123_MFI CD123_CV CD123_MAD CD63_MFI CD63_CV CD63_MAD CD63-CD203+ CD63-CD203+_CD69_CV CD63-CD203+_CD69_MFI CD63-CD203+_CD69_MAD CD63-CD203+_CD63_CV CD63-CD203+_CD63_MFI CD63-CD203+_CD63_MAD CD63-CD203+_CD203_CV CD63-CD203+_CD203_MFI CD63-CD203+_CD203_MAD CD63-CD203+_CD123_CV CD63-CD203+_CD123_MFI CD63-CD203+_CD123_MAD CD63-CD203+_HLADR_MFI CD63+CD203+ CD63+CD203+_CD69_CV CD63+CD203+_CD69_MFI CD63+CD203+_CD69_MAD CD63+CD203+_CD63_CV CD63+CD203+_CD63_MFI CD63+CD203+_Cd63_MAD CD63+CD203+_CD203_CV CD63+CD203+_CD203_MFI CD63+CD203+_CD203_MAD CD63+CD203+_CD123_CV CD63+CD203+_CD123_MFI CD63+CD203+_CD123_MAD CD63+CD203+_HLADR_MFI CD63+CD203- CD63-CD203- CD63-CD203-_CD69_CV CD63-CD203-_CD69_MFI CD63-CD203-_CD69_MAD CD63-CD203-_CD63_CV CD63-CD203-_CD63_MFI CD63-CD203-_CD63_MAD CD63-CD203-_CD203_CV CD63-CD203-_CD203_MFI CD63-CD203-_CD203_MAD CD63-CD203-_CD123_CV CD63-CD203-_CD123_MFI CD63-CD203-_CD123_MAD CD63-CD203-_HLADR_MFI CD63-CD69+ CD63-CD69+_CD69_CV CD63-CD69+_CD69_MFI CD63-CD69+_CD69_MAD CD63-CD69+_CD63_CV CD63-CD69+_CD63_MFI CD63-CD69+_CD63_MAD CD63-CD69+_CD203_CV CD63-CD69+_CD203_MFI CD63-CD69+_CD203_MAD CD63-CD69+_CD123_CV CD63-CD69+_CD123_MFI CD63-CD69+_CD123_MAD CD63+CD69+ CD63+CD69+_CD69_CV CD63+CD69+_CD69_MFI CD63+CD69+_CD69_MAD CD63+CD69+_CD63_CV CD63+CD69+_CD63_MFI CD63+CD69+_CD63_MAD CD63+CD69+_CD203_CV CD63+CD69+_CD203_MFI CD63+CD69+_CD203_MAD CD63+CD69+_CD123_CV CD63+CD69+_CD123_MFI CD63+CD69+_CD123_MAD CD63+CD69- CD63+CD69-_CD69_CV CD63+CD69-_CD69_MFI CD63+CD69-_CD69_MAD CD63+CD69-_CD63_CV CD63+CD69-_CD63_MFI CD63+CD69-_CD63_MAD CD63+CD69-_CD203_CV CD63+CD69-_CD203_MFI CD63+CD69-_CD203_MAD CD63+CD69-_CD123_CV CD63+CD69-_CD123_MFI CD63+CD69-_CD123_MAD CD63-CD69- CD63-CD69-_CD69_CV CD63-CD69-_CD69_MFI CD63-CD69-_CD69_MAD CD63-CD69-_CD63_CV CD63-CD69-_CD63_MFI CD63-CD69-_CD63_MAD CD63-CD69-_CD203_CV CD63-CD69-_CD203_MFI CD63-CD69-_CD203_MAD CD63-CD69-_CD123_CV CD63-CD69-_CD123_MFI CD63-CD69-_CD123_MAD 1: 1 RPMI pre 1319 0.94 62.4 411 652 1196 130 132 13496 36.6 147 487 252 80.9 47.1 441 68.4 526 47 513 85.2 62.5 2047 138 34.4 13961 147 220 1.36 99.3 1374 144 66.3 10573 145 80.8 9565 146 69.1 16829 147 2589 0 51.6 281 49 1444 34.5 430 76.5 34.7 704 125 35.5 13009 147 238 21.7 186 701 158 42.9 475 82 96.3 1182 133 38.2 13889 147 1.14 74.7 3516 145 71.6 10205 145 68.8 12441 147 58.9 20642 147 0.45 2871 -9.87 -275 67.4 5950 141 66.2 4084 143 26.4 9300 147 76.7 2426 -5.08 -346 33.4 464 79.8 77.3 1153 132 33.9 13332 147 2: 2 fMLP pre 1139 0.96 74 332 515 1925 100 138 14338 33.3 147 647 264 82.3 59.8 285 61.8 636 93.1 535 82.6 69.1 2701 140 31.7 14415 147 166 10 261 180 208 64.7 12450 145 49.6 7323 146 41.3 13883 147 178 0.088 30.1 243 70.2 712 35.9 375 70.6 38.5 656 123 33.5 14367 147 510 21.8 101 704 156 54.6 493 84 97 1581 136 32.6 15108 147 5.18 165 985 151 67.6 12509 145 50.8 7447 146 46.7 14724 147 7.64 1600 -3.21 -377 84.1 7470 142 52.6 6193 145 29.3 12515 147 65.4 5002 -7.42 -326 56.6 436 76.5 88.2 1619 136 31.8 14287 147 3: 3 IgE1 pre 1141 0.68 72.1 332 449 1447 117 134 14009 35.5 147 436 256 69.1 52.5 377 62.8 607 75.1 476 76.2 76.9 2764 140 33.8 13986 147 222 2.1 117 775 144 48 10326 144 52.1 8714 146 46.6 15269 147 1341 0 45.4 230 71.4 455 40.2 343 62.2 36 648 122 36.6 13980 147 606 22.7 123 778 151 53.3 428 69.7 112 1243 131 34.5 15530 147 1.67 91.3 2462 145 54 8244 143 56.7 8371 146 49.2 15405 147 1.31 -1317 -16.5 -252 87.4 5897 138 51.4 7787 146 36.5 14433 147 74.3 1752 -2.59 -339 50.1 395 67.3 106 1417 134 34.6 13540 147 4: 4 IgE2 pre 1172 0.98 57 398 941 1248 122 133 14089 34.9 147 360 320 57.8 49.1 220 58.9 521 57 372 62.1 74.2 2148 138 33.8 14492 147 152 1.71 106 1445 146 55.3 11103 144 50 10297 146 48.8 18036 147 2868 0 49.1 315 44.5 12614 33.2 312 54.4 34.8 686 124 34.2 13581 147 297 19.5 80.5 643 154 40.4 380 64.2 107 1193 133 31.8 14522 147 1.37 83.9 3689 146 53 11605 144 44.9 11603 146 45 20087 147 0.43 3754 -15.7 -237 80.5 7747 143 67.2 4864 144 21.5 10993 147 78.7 1599 -2.87 -299 43.3 331 56 96.1 1206 132 34.7 13917 147 5: 5 IgE3 pre 1206 0.91 78.8 275 364 1245 121 133 14743 35.5 147 509 240 84.7 51.1 259 85.7 320 50.6 535 87.9 78.3 2111 138 33.8 15341 147 209 1 95.6 717 145 71.3 10723 144 73.6 9194 146 58.4 16193 147 1766 0 47.9 260 66.8 576 35.8 455 79.9 36.6 691 125 36.2 14104 147 360 25.4 105 725 155 38.1 495 86.2 110 1144 133 34.8 15963 147 0.75 60.2 3375 145 84.3 9242 144 80 9566 146 56.5 17265 147 0.66 -335 -40.7 -257 102 5152 140 69.1 6446 146 49.9 13208 147 73.2 1023 1.76 -387 38.4 490 82.7 92.8 1238 133 34.7 14333 147 6: 6 RPMI post 1181 0.87 41.5 390 -910 990 135 129 13301 33.7 147 444 263 74.7 34.5 411 45.2 1395 53.2 471 79.1 63.2 1941 137 31.6 14086 147 181 0.85 79.7 1385 146 69.6 11710 145 67.9 11281 147 49.1 20173 147 7584 0 64.6 319 35.4 -573 37.7 413 73.2 35.9 677 123 33.3 12829 147 190 18.2 124 708 158 39.7 459 78.9 94.8 969 129 31.2 13825 147 0.76 61.3 3979 146 72.9 10016 144 68.4 12288 147 55.1 19125 147 0.25 904 -2.32 -254 116 7809 142 98.8 5091 145 39.8 15600 147 80.8 -2029 -15.3 -261 38.1 425 74 85.9 968 128 33.3 13134 147 7: 7 fMLP post 1104 0.85 68.6 312 587 2057 98.4 139 14112 33.5 147 684 262 89.1 62.9 353 49.7 4259 78.1 583 90.1 69 2841 141 33.5 14206 147 145 8.61 171 266 196 60.7 12693 145 44.9 8182 146 34 14816 147 378 0 28.5 256 72.2 675 36.6 426 79.7 36.1 692 124 32.9 13705 147 354 20.7 112 717 169 46.2 543 90.2 89.3 1537 136 33.2 14913 147 4.89 105 1484 147 65.7 11797 145 44.4 8552 146 30 16106 147 6.07 476 16.2 2572 84 7567 143 45.9 6864 145 35.4 13271 147 68.3 -3198 -13.6 -294 49.4 496 85.4 89.8 1831 137 33.3 13823 147 8: 8 IgE1 post 1040 0.7 76.1 429 453 1456 130 132 14632 36.4 147 614 201 90.2 50.5 553 62.9 639 45.2 657 95.3 89.4 3011 141 34.1 14963 147 171 2.4 84.1 1034 144 40 10360 144 53 8979 146 46.4 15851 147 2665 0 47.1 268 76.1 425 37.1 500 82.7 39.7 629 120 38 14226 147 569 24.1 223 753 155 47.5 546 89.2 132 1223 129 34.7 15904 147 2.02 94.1 3649 145 43.5 9111 144 59.3 9872 146 50.3 15709 147 0.87 639 8.17 -735 81.7 6209 141 55.6 6931 144 30 13383 147 73 2717 -5.76 -357 39 580 90 121 1439 132 35.9 14221 147 9: 9 IgE2 post 1134 0.95 54 404 2002 1348 112 132 14945 35.1 147 569 181 89.1 51.3 530 42.6 -925 41.9 608 93.1 72.7 2431 138 34 15533 147 176 0.79 79.8 1916 145 50.8 11535 145 45.8 12513 147 39 19136 147 7231 0 47.9 276 60.9 867 33 506 84.9 34.6 704 124 35.6 14281 147 282 21.3 170 738 167 39.3 539 89.7 107 1177 131 35.7 15968 147 0.88 74.2 2809 145 61.3 9537 145 58.2 10305 147 45.8 16831 147 0.088 • -31.4 -599 • 5099 140 • 9006 146 • 23042 147 77.8 -3045 -12.9 -321 35.1 559 88.8 98.4 1365 133 34.4 14650 147 10: 10 IgE3 post 1206 0.94 59.7 479 1490 1155 116 131 15376 33.1 147 526 130 87.5 46.2 539 62.6 781 40.8 564 91 83.1 1996 137 31.6 15924 147 191 0.58 89 1031 145 42.6 8996 144 79.5 8581 146 37.8 13768 147 3496 0 53.2 273 54.2 2614 34.7 480 83.2 34 712 125 34.1 14934 147 265 22.4 223 738 163 38 535 89.5 104 1175 132 33.2 16061 147 0.58 61.6 2547 145 51.8 5693 141 60.3 6154 145 41 12601 147 0.17 296 49.2 479 15.6 14504 146 110 9868 146 35.3 15156 147 76.9 8116 -8.97 -328 33.2 511 86.7 103 1130 131 32.8 15205 147 11: 11 RPMI 4-hour 1152 0.83 43.3 423 -657 890 155 125 13472 34.7 147 499 250 78.2 29.5 402 46.3 -661 44.1 538 82.6 44.7 1726 134 30.3 14303 147 159 0.78 54.7 6198 147 58.3 14034 145 52.7 15930 147 49.8 25671 148 16487 0 69.7 337 37.1 -461 33 466 76 37.1 656 119 33.6 13040 147 208 20.2 126 670 162 34.3 526 84.6 75.6 858 125 35.2 13777 147 0.95 62.9 4777 147 73.2 10359 145 69.1 11312 147 58 21845 148 0 • • • • • • • • • • • • 78.8 -938 -20.5 -216 33.3 475 76.5 65.9 872 125 32.1 13316 147 12: 12 fMLP 4-hour 1255 0.94 43 412 -1376 1428 115 133 13358 35 147 546 277 79.6 50.7 364 27.3 -486 94 505 82.1 72.1 2201 137 34.5 13258 147 133 6.29 181 264 199 52.9 12350 145 54.6 6923 145 44.4 14455 147 455 0 43 279 40.8 -1362 46.9 392 73.4 34.5 697 122 33.5 13321 147 227 17.2 71.6 632 170 47.4 460 80.4 78.3 1146 131 31.4 14253 147 3.27 106 1790 144 55 11544 145 55.7 7908 145 47.2 16200 147 4.86 3233 -4.8 -484 78.6 7774 142 51.4 5400 145 30.5 12507 147 74.7 -949 -19.5 -273 50.9 427 77.2 93 1284 132 34.4 13105 147 13: 13 IgE1 4-hour 951 0.68 70.6 387 896 1603 123 131 17125 36.5 147 671 238 93.3 56 462 57.2 -1781 48.8 721 96.8 89 2986 138 34.5 17381 147 240 1.89 137 594 154 60 12664 145 50.1 8984 146 39.9 17737 147 1140 0.11 42 234 78.6 466 38.5 535 86.7 38.2 666 117 38.9 16791 147 690 25.3 183 918 163 45.1 628 94.7 131 1231 127 38.9 18541 147 1.37 102 2256 144 56.3 9437 145 51.5 9188 146 41.5 17980 147 1.58 212 50 335 108 6377 142 54.6 7251 145 26.7 17268 147 71.7 -1164 -20 -316 41 625 92 117 1650 132 34.8 16633 147 14: 14 IgE2 4-hour 1170 0.92 43 405 -459 1130 131 130 13280 34.6 147 419 250 70.9 42.5 496 35.6 -427 62.4 459 78.1 71.1 2244 138 33.3 13842 147 135 1.28 63.8 2784 146 59.5 10036 144 58.3 11912 146 56.2 17986 147 8112 0 56.2 257 40 -447 32.9 365 66.3 37.7 649 122 33.2 12782 147 181 20 140 661 166 40.2 443 75.3 113 1064 129 33.1 13980 147 1.37 65.6 3372 146 67.3 8639 144 67 10221 146 58.4 17143 147 0.43 110 107 260 38.8 3275 136 70.7 4520 144 47.5 12301 147 78.2 -835 -22.3 -230 39.7 388 68.9 98.5 1097 130 33.3 13054 147 15: 15 IgE3 4-hour 1140 0.89 37.6 357 -412 1064 123 127 15181 33.8 147 520 206 80.4 41.9 360 28.2 -338 46.4 555 83.2 71.3 1885 135 31.9 16010 147 170 0.88 101 1008 146 69 9689 143 83.1 8796 144 74.2 15038 147 3911 0 57.2 269 40.3 -466 34.5 476 77.6 34.5 686 119 33.6 14602 147 249 19.3 68.3 669 169 36 528 83.2 88.1 903 124 29.1 16484 147 0.79 71.1 3225 146 78.5 8595 141 82 9160 144 84.1 13777 146 0.35 295 38.2 592 102 4999 137 35.2 5147 144 37.4 15463 147 79.6 -772 -22.9 -238 35.5 501 79.3 91.1 1078 127 33.9 14893 147 Mean 1154 0.87 58.8 383 302 1345 122 132 14364 34.8 147 529 239 80.5 48.4 403 53 338 58.5 539 84.4 72.4 2336 138 33.1 14780 147 178 2.7 115 1398 157 57.9 11283 144 59.7 9812 146 49 16989 147 4013 0.013 48.9 273 55.8 1169 36.3 432 75.2 36.2 677 122 34.8 13970 147 348 21.3 136 717 161 42.9 499 82.8 102 1175 131 33.8 15248 147 1.8 85.3 2928 146 63.7 9769 144 61.1 9626 146 51.2 17029 147 1.68 980 10.3 54.9 80.5 6845 141 63.7 6389 145 34.3 14174 147 75.2 647 -11.9 -302 41.1 474 78.7 94.9 1290 132 33.9 14096 147 StdDev 85.3 0.1 14.3 52.3 912 325 13.9 3.59 1035 1.16 0 95.2 44.9 9.46 8.76 102 15.9 1358 18.1 84.2 8.79 11.1 423 1.96 1.33 1078 0 32.7 3.03 53.8 1493 23 9.66 1367 0.64 13.4 2304 0.76 11.4 3078 0.26 4326 0.035 11 30.6 15.7 3306 3.66 64.7 8.89 1.74 24.8 2.49 1.87 1027 0 165 2.41 50.7 69.3 6.23 6.02 60.6 8.2 16.5 196 3.52 2.6 1306 0 1.47 27.6 1015 1.7 11.4 1716 1.36 11.4 1803 0.85 12.1 2569 0.38 2.43 1483 39.2 829 27.6 2581 2.62 20.8 1700 0.95 8.2 3261 0 4.33 3078 8.06 49.7 7.39 77.6 9.67 13.9 266 3.16 1.1 975 0