LOCUS                  New_DNA        2967 bp ds-DNA   linear       28-JAN-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1857..2717)
                     /label=AmpR
                     /ApEinfo_fwdcolor=#f8c887
                     /ApEinfo_revcolor=#f8c887
     misc_feature    901..906
                     /label=BamHI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    916..921
                     /label=PstI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    2958..2963
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#0080ff
     misc_feature    772..900
                     /label=dblTerm
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    6..59
                     /label=P_tet of R0040
                     /ApEinfo_fwdcolor=#dfd0df
                     /ApEinfo_revcolor=#dfd0df
     misc_feature    910..915
                     /label=XhoI
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    85..762
                     /label=mRFP of Bca1144#5
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
     misc_feature    1080..1762
                     /label=colE1 ori
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
     misc_feature    complement(979..998)
                     /label=G00101
                     /ApEinfo_fwdcolor=#99e4fb
                     /ApEinfo_revcolor=#99e4fb
     misc_feature    2897..2918
                     /label=CA998
                     /ApEinfo_fwdcolor=#9bd1f9
                     /ApEinfo_revcolor=#9bd1f9
ORIGIN
        1 GATCTtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcacG
       61 GATCTGAAAG AGGAGAAAGG ATCTatggcg agtagcgaag acgttatcaa agagttcatg
      121 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      181 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      241 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      301 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      361 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      421 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      481 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
      541 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
      601 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
      661 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
      721 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataaGGATC Tccaggcatc
      781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      841 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      901 GGATCCtaaC TCGAGctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1081 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1501 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2821 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2881 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2941 gctaaggatg atttctggaa ttcatgA   
//