Num	Seq_name	left_primer	right_primer	left_tm	right_tm	left_gc	right_gc	amplicon_size
1	>GRC13_0019_FC:7:1:11327:10785#AGCT	TCTCAAAATCCTCCTCCTC	ATCACCCCTAGACTTGGTT	54.214	53.444	47.368	47.368	126
2	>GRC13_0019_FC:7:1:7443:11171#AGCT	GTCAACAGCACCGTCAC	GTTTCAGGTTGTGGTTCAC	53.816	53.629	58.824	47.368	110
3	>GRC13_0019_FC:7:1:3526:20003#TCCC	AAGCTCTCCCAATTCTCC	AACCCTGGTCTCTCAACTT	54.225	53.615	50.000	47.368	146
4	>GRC13_0019_FC:7:2:15944:6758#AGCT	CAACAACAACAACTCCACA	GAGGAGAGTGATCGGTTCT	53.579	54.103	42.105	52.632	145
5	>GRC13_0019_FC:7:3:12401:3427#AGCT	GGTCTCCGATCTAAGGATT	ACCAGAGTGAACCAAAACN	53.177	54.162	47.368	44.444	137
6	>GRC13_0019_FC:7:4:1485:6821#AGCT	GTTGGGATCATCAGAATCA	TCAACAGGGCATATATTGAA	54.005	54.136	42.105	35.000	147
7	>GRC13_0019_FC:7:5:11694:14121#AGCT	GTTTCACGCACTTTAATGG	TTTCACGAAATCATCATCA	54.249	53.140	42.105	31.579	152
8	>GRC13_0019_FC:7:7:18222:3474#AGCT	CATCATCATCACTTACGGC	TTGTTGTTGTTGATGTTGC	54.852	53.716	47.368	36.842	113
9	>GRC13_0019_FC:7:7:15436:12359#AGCT	TAAGGGAGAAAGAGAGGGN	TCTCTACCACAACCACCAC	54.682	54.116	50.000	52.632	123
10	>GRC13_0019_FC:7:8:16663:4058#AGCT	AATGAACAGTGCCACAAAT	GTTGTTGTTGTTGGTGGTT	53.820	54.068	36.842	42.105	131
11	>GRC13_0019_FC:7:8:12159:4669#AGCT	ATTACTGAAACCAACACGG	GGACCTCGTGGACCTTA	53.446	54.073	42.105	58.824	169
12	>GRC13_0019_FC:7:10:11570:10032#AGCT	TTATTGAAGACGAGCAAGG	CTTCTTCTTCACTCGACCC	54.035	54.862	42.105	52.632	104
13	>GRC13_0019_FC:7:11:7469:13116#CATC	CCTACTCAGTGTTTGAGGC	GAACCACACTCAAAGCAAC	53.227	54.007	52.632	47.368	193
14	>GRC13_0019_FC:7:14:10302:6524#GCCA	AATCAAAATGGCTAATCCC	GAGATTGCTTTACGTGGTG	54.150	54.202	36.842	47.368	134
15	>GRC13_0019_FC:7:19:2519:17074#GGAG	GTGGTGGTGGAGACTTGTA	CCCAATCCATACAAAGGA	54.116	54.109	52.632	44.444	191
16	>GRC13_0019_FC:7:21:6888:14599#GCAG	ATGCATTATTGCACGAGTT	AAGGTAGTTCCTCAGCGAT	54.177	53.974	36.842	47.368	125
17	>GRC13_0019_FC:7:21:8500:18506#GGTG	AACTTGCTTCAACACCTTG	AAGAAGAGCGTGGAGACTT	53.800	54.154	42.105	47.368	153
18	>GRC13_0019_FC:7:22:8478:12553#GGAA	ACGCTTGTTTTGAAGAATG	TTGAAGAGGAGAAAGGTGN	53.939	53.969	36.842	44.444	110
19	>GRC13_0019_FC:7:23:3900:13924#TGCC	GATCACCCCCTACTACACC	TACACGAGACAAAAGCCAG	54.155	54.912	57.895	47.368	165
20	>GRC13_0019_FC:7:23:4327:20090#AGCT	CAGAGTTGAGTTCATGGGA	TGTAGTCCTCCTCCTCCTC	54.371	54.121	47.368	57.895	138
21	>GRC13_0019_FC:7:27:9123:4891#AGCT	GATTGTTGAGGTTGTGACC	GATAACGTGCCAAATGAAG	53.520	54.154	47.368	42.105	134
22	>GRC13_0019_FC:7:31:2453:1643#TGTC	GACGTGCCGAAATGTATAG	GGGGGTACATATGCAAGA	54.193	54.133	47.368	50.000	177
23	>GRC13_0019_FC:7:32:14711:3994#AGCT	CAACTGCTCCTAACCCAG	CTGCCTTCTTCTTAGGCTT	54.157	54.081	55.556	47.368	109
24	>GRC13_0019_FC:7:40:15576:4770#AGCT	TGACAAAGCCAAACTCAAC	GATGCAGCGCTTCTTAG	54.678	52.934	42.105	52.941	164
25	>GRC13_0019_FC:7:41:8251:9707#GCCC	CCCAGGAGTATTTAGCCTT	CATCTGGGTTGTCATGATT	53.580	53.963	47.368	42.105	176
26	>GRC13_0019_FC:7:41:9829:20457#AGCT	CTTATCCGTTGAACAATGC	AACAACAACAACAACCACC	54.154	54.068	42.105	42.105	138
27	>GRC13_0019_FC:7:43:15416:14979#AGCT	AGATAGGGTGCTGTTTGNN	ATGTGAAATGGCTGAGAGA	54.378	54.006	47.059	42.105	139
28	>GRC13_0019_FC:7:44:9129:5500#CGCC	GGGACACTTGTTTTGAAGA	TTGAAGAGGAGAAAGGTGN	53.470	53.969	42.105	44.444	128
29	>GRC13_0019_FC:7:46:2837:8473#CTAT	GACCGAACCAACAGAAATA	GGAGAGAAGGGAGTCCAC	53.481	54.333	42.105	61.111	126
30	>GRC13_0019_FC:7:49:18650:1498#CTTC	CGACATCATGGTAAGATCC	TAGCCATTGCTGTGCTAGT	53.631	54.597	47.368	47.368	124
31	>GRC13_0019_FC:7:51:1708:10097#AGCT	ACACAAACCCTACTCCTCC	ATTAGGGCTTTGTTGGAAG	53.935	54.467	52.632	42.105	153
32	>GRC13_0019_FC:7:54:15851:14941#CGGA	AAGTGTGGTGGGTAGTGTG	GGCTAGCCTTCTCTTCTCA	54.073	54.874	52.632	52.632	171
33	>GRC13_0019_FC:7:55:2851:6269#AGCT	TTGAAGAGGAGAAAGGTGN	ACGCTTGTTTTGAAGAATG	53.969	53.939	44.444	36.842	105
34	>GRC13_0019_FC:7:56:7737:9500#AGCT	GGCTGTTCGTTCTGTACC	TCACAAAGCCTTGCTTAAC	53.934	54.114	55.556	42.105	169
35	>GRC13_0019_FC:7:56:7568:18864#AGCT	TTGAAGAGGAGAAAGGTGN	ACGCTTGTTTTGAAGAATG	53.969	53.939	44.444	36.842	118
36	>GRC13_0019_FC:7:57:9277:4513#AGCT	TCCACTGCTTCTTTTTCAG	TCCAACAACAACAAAGACC	54.039	54.313	42.105	42.105	128
37	>GRC13_0019_FC:7:58:8462:6914#AGCT	AATCAAGTGCAATGCTTGT	CTGACCTCCAATCTTGATG	54.185	53.835	36.842	47.368	131
38	>GRC13_0019_FC:7:59:2740:5919#ATCG	GGGACACTTGTTTTGAAGA	TTGAAGAGGAGAAAGGTGN	53.470	53.969	42.105	44.444	126
39	>GRC13_0019_FC:7:60:10521:14930#AGCT	GAAGACGAAGACGACGAC	GATGAGGTCGTTAGGGTTT	54.022	54.069	55.556	47.368	160
40	>GRC13_0019_FC:7:61:18614:12260#GTGA	CGTCCATTTCTTCATCATC	ATATAGGAGCCCGTAAAGG	53.775	53.587	42.105	47.368	145
41	>GRC13_0019_FC:7:62:1704:7950#CTCT	CTTCTTCACCCACCATAAA	CCACAAGAGCAGAAGAAGA	53.501	53.983	42.105	47.368	181
42	>GRC13_0019_FC:7:63:2252:18958#AGCT	CTACTCCTGTGACACCACC	GTGAAGAAGAAGGAGGAGG	53.622	53.853	57.895	52.632	137
43	>GRC13_0019_FC:7:64:16852:15530#AGCT	AGAAGAAAATGGCAACACA	CCACTGAAGAGGTCAGAGA	54.161	53.531	36.842	52.632	153
44	>GRC13_0019_FC:7:66:19657:3891#CGCC	ACGCTTGTTTTGAAGAATG	TTGAAGAGGAGAAAGGTGN	53.939	53.969	36.842	44.444	117
45	>GRC13_0019_FC:7:67:5853:5710#AGCT	ATACATCACCAGCAGCAAT	GCCAGTGATTCAGAGAGTG	53.944	54.054	42.105	52.632	152
46	>GRC13_0019_FC:7:68:7642:17754#AGCT	AAGAAGGAAGAGCTTTTGG	ATTGTCCTTCAAATACGCA	53.874	54.154	42.105	36.842	130
47	>GRC13_0019_FC:7:73:11878:9246#GAGG	TTTGGAAGGTGTAGTTGCT	CAACCGAGTACTGTGGAAN	53.307	53.972	42.105	50.000	103
48	>GRC13_0019_FC:7:77:4837:7170#GTTG	TAGCTGTTGCAATATCAGC	ACAACAACATCAACAGCNN	52.487	54.067	42.105	41.176	134
49	>GRC13_0019_FC:7:78:15462:6290#GGAG	ATTAGGGCTTTGTTGGAAG	ACACAAACCCTACTCCTCC	54.467	53.935	42.105	52.632	153
50	>GRC13_0019_FC:7:79:14541:19092#GGTA	CTGTTTGGATCTGAGCTTC	TCATGATGATGATGGTGG	53.261	53.916	47.368	44.444	159
51	>GRC13_0019_FC:7:81:12761:19457#AGCT	ACCATATCCAACTACAGACAAA	GATGACGATATGGGTTTCA	53.887	54.103	36.364	42.105	134
52	>GRC13_0019_FC:7:82:12708:17547#AGCT	ACTTGCAGCATCTTCTGTT	CCTTTGTGTTGGAGCTAGA	53.393	53.899	42.105	47.368	148
53	>GRC13_0019_FC:7:87:18663:10783#AGCT	TTGGTTATCCATTTTGGTG	AGAACCATTTCCCATTCTC	54.267	53.932	36.842	42.105	145
54	>GRC13_0019_FC:7:87:14568:12392#AGCT	TTGAAGAGGAGAAAGGTGN	GGGACACTTGTTTTGAAGA	53.969	53.470	44.444	42.105	101
55	>GRC13_0019_FC:7:88:7373:20603#TTGG	AATGCTTCAGCTGACAATG	CATTTTCTCATCATGCTCC	54.805	53.961	42.105	42.105	185
56	>GRC13_0019_FC:7:89:3412:13602#AGCT	GTTGAAACTGCAAAGCAAT	CAGGTCTCTTTCCAACCTT	53.921	54.269	36.842	47.368	174
57	>GRC13_0019_FC:7:90:2088:4446#GTCG	TCGTCGAACTTGTCCTTAC	ATGCTCGGTATCACTCCTC	53.698	55.113	47.368	52.632	131
58	>GRC13_0019_FC:7:92:14352:1328#AGCT	GTGCTTGAGGACGAAGAT	ATCCTTGTCACCATCATCA	53.487	54.400	50.000	42.105	133
59	>GRC13_0019_FC:7:96:6790:13525#GGAG	GTGGTGGTGGAGACTTGTA	CCCAATCCATACAAAGGA	54.116	54.109	52.632	44.444	191
60	>GRC13_0019_FC:7:96:11541:18845#GCCA	CCATTACCCTGAAGTTCCT	CTGCTCCAGTGATAGATGG	54.086	54.067	47.368	52.632	173
61	>GRC13_0019_FC:7:97:18802:11860#AGCT	TCATCATCATCATCACCCT	CTACATTGAACGTGATGGC	54.295	54.946	42.105	47.368	181
62	>GRC13_0019_FC:7:98:17233:11439#AGCT	CTTCATCAACAACCCATGT	GTGAGAGCCCTGTTATCCT	54.067	54.195	42.105	52.632	110
63	>GRC13_0019_FC:7:99:4423:7737#AGCT	TGGAGGCTCATTTGAAGTA	AACAGCAACAACAACTGCT	54.261	54.334	42.105	42.105	143
64	>GRC13_0019_FC:7:105:17050:15904#AGCT	GGCTGTTCGTTCTGTACC	TCATCATCATCGCATACAA	53.934	54.141	55.556	36.842	188
65	>GRC13_0019_FC:7:106:8714:11717#CTAA	CAACAAAATTCGTTCCGTA	GCTCTGCTTACATTGGTGT	54.702	53.721	36.842	47.368	173
66	>GRC13_0019_FC:7:108:17098:10659#CTGA	TGGTGAGTTCATGTTGTTG	CATTCCCTCACTCACAAAG	53.465	53.941	42.105	47.368	187
67	>GRC13_0019_FC:7:110:13272:10575#TTGG	AAAGTGTTTGTTGTGGTGG	CATTTTCTCATCATGCTCC	54.269	53.961	42.105	42.105	151
68	>GRC13_0019_FC:7:115:17780:12075#AGCT	CGCATTCCTGCTGAANN	CTATCACCATCACCACAGC	56.808	54.734	53.333	52.632	158
69	>GRC13_0019_FC:7:116:13867:13874#AGCT	ATTACTGAAACCAACACGG	GGACCTCGTGGACCTTA	53.446	54.073	42.105	58.824	183
70	>GRC13_0019_FC:7:117:7768:8100#AGCT	TCACGCATCTTTTTCTTCT	GATTGGGGATACGGCGG	53.523	62.222	36.842	64.706	138