LOCUS New_DNA 4832 bp ds-DNA linear 05-FEB-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 4621..4638 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(2592..2612) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 4654..4753 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2429..2448) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4762..4783 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2456..2555 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2824..3834 /label=specR /ApEinfo_fwdcolor=#f2f09d /ApEinfo_revcolor=#f2f09d /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3848..4514 /label=colE1 /ApEinfo_fwdcolor=#c0c0c0 /ApEinfo_revcolor=#c0c0c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2572..2591) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 377..1006 /label=toxR> /ApEinfo_fwdcolor=#9f009f /ApEinfo_revcolor=#9f009f /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2351..2356 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4823..4828 /label=EcoRI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1793..1798 /label=PstI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3837..3842 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2684..2689 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(3166..3171,3186..3187) /label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(4028..4029,4044..4049)) /label=AcuI/Eco57I(1) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 728..733 /label=MfeI /ApEinfo_fwdcolor=#8fc2c1 /ApEinfo_revcolor=#8fc2c1 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1034..2327 /label=mukF /ApEinfo_fwdcolor=#9b73dd /ApEinfo_revcolor=#9b73dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6..345 /label=P_rffGH /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=#ff8080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 377..451 /label=ToxR prepro /ApEinfo_fwdcolor=#7d0d82 /ApEinfo_revcolor=#7d0d82 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 452..1006 /label=ToxR active peptide /ApEinfo_fwdcolor=#8b52ad /ApEinfo_revcolor=#8b52ad /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GATCTCTCAT TAATAAACTG GCAATGACCA AGACCAATGA CGATTTCTTC GAAATGATGA 61 AACGCTCATA AATTTGTCTT ATGCCAAAAA CGCCACGTGT TTACGTGGCG TTTTGCTTTT 121 ATATCTGTAA TCTTAATGCC GCGCTGGCGA TGTTAGGAAA ATTCCTGGAA TTTGCTGGCA 181 TGTTATGCAA TTTGCATATC AAATGGTTAA TTTTTGCACA GGACTGGTGG GTTTGGAACG 241 GACTTTCCCT TCTGAATAAA GGTCTTCGTG GTTATACTTC TGCTAATAAT TTTCTCTGAG 301 AGCATGCATT GTGAATTTAC TGACAGTGAG TACTGATCTC ATCAGGGATC TGCCACGCAC 361 AAAACTACGG TAGCAAATGT TCGGATTAGG ACACAACTCA AAAGAAATCT CGATGAGTCA 421 TATTGGTACT AAATTCATTC TTGCTGAAAA ATTTACCTTC GATCCCCTAA GCAATACTCT 481 GATTGACAAA GAAGATAGTG AAGAGATCAT TCGATTAGGC AGCAACGAAA GCCGTATCCT 541 TTGGCTGCTG GCCCAACGTC CAAACGAGGT AATTTCTCGC AATGATTTGC ATGACTTTGT 601 TTGGCGAGAG CAAGGTTTTG AAGTCGATGA TTCCAGCTTA ACCCAAGCCA TTTCGACTCT 661 GCGCAAAATG CTCAAAGATT CGACAAAGTC CCCACAATAC GTCAAAACGG TTCCGAAGCG 721 CGGTTACCAA TTGATCGCCC GAGTGGAAAC GGTTGAAGAA GAGATGGCTC GCGAAAACGA 781 AGCTGCTCAT GACATCTCTC AGCCGGAGTC CGTCAATGAA TACGCAGAAT CAAGCAGTGT 841 GCCTTCATCT GCTACCGTAG TGAACACACC GCAGCCAGCC AATGTCGTGG CGAATAAATC 901 GGCTCCAAAC TTGGGGAATC GACTGTTTAT TCTGATAGCG GTCTTACTTC CCCTCGCAGT 961 ATTACTGCTC ACTAACCCAA GCCAATCCAG CTTTAAACCC CTAACGGGAT CTgctagcGG 1021 CAGTGGATCT agtgaatttt cccagacagt cCCCGAACTG GTTGCCTGGG CCAGAAAAAA 1081 TGACTTCTCC ATCTCGCTGC CGGTAGACCG ACTCTCTTTT CTGCTGGCGG TTGCCACGCT 1141 GAACGGCGAG CGTCTGGATG GTGAGATGAG TGAAGGCGAG CTGGTGGATG CATTCCGCCA 1201 TGTGAGTGAT GCGTTTGAGC AAACCAGCGA AACCATCGGC GTGCGCGCCA ATAACGCGAT 1261 CAACGACATG GTGCGTCAAC GTCTGCTGAA CCGCTTTACC AGCGAGCAGG CGGAAGGGAA 1321 CGCAATTTAC CGTCTGACGC CGCTCGGCAT CGGCATTACT GACTACTACA TCCGTCAGCG 1381 CGAGTTTTCT ACGCTGCGTC TTTCTATGCA GTTGTCGATT GTGGCGGGTG AGCTCAAACG 1441 CGCAGCAGAT GCCGCCGAAG AGGGCGGTGA TGAATTTCAC TGGCACCGTA ATGTCTATGC 1501 GCCACTGAAA TATTCGGTAG CAGAAATTTT CGACAGTATC GACCTGACGC AACGTCTGAT 1561 GGACGAACAG CAGCAGCAGG TGAAGGACGA TATCGCCCAG TTGCTGAACA AAGACTGGCG 1621 GGCGGCGATT TCCAGCTGTG AATTGTTGCT TTCGGAAACT TCCGGAACGC TGCGTGAATT 1681 GCAGGATACG CTGGAAGCGG CAGGCGACAA ATTGCAGGCT AATCTGTTGC GCATTCAGGA 1741 TGCGACGATG ACCCATGACG ATCTGCATTT CGTCGATCGT CTGGTGTTCG ATCTGCAGAG 1801 CAAACTCGAT CGTATTATCA GTTGGGGCCA GCAATCCATC GACTTGTGGA TTGGCTACGA 1861 CCGCCACGTA CACAAATTTA TTCGTACCGC GATCGATATG GATAAAAACC GCGTCTTTGC 1921 TCAGCGGTTA CGTCAGTCGG TACAAACCTA TTTTGATGAG CCGTGGGCGC TAACTTATGC 1981 CAATGCCGAT CGTCTGCTGG ATATGCGTGA CGAAGAGATG GCACTGCGCG ATGAAGAAGT 2041 GACTGGGGAA CTTCCTGAGG ATCTGGAATA CGAAGAGTTT AACGAGATCC GCGAACAGCT 2101 GGCGGCGATC ATCGAAGAAC AACTTGCCGT GTACAAAACC AGACAAGTGC CGCTGGATCT 2161 TGGTCTGGTG GTACGCGAAT ATCTGTCACA GTATCCGCGT GCACGTCACT TTGACGTTGC 2221 GCGTATTGTT ATTGATCAGG CGGTACGTCT TGGCGTAGCG CAAGCAGATT TCACCGGACT 2281 GCCAGCGAAA TGGCAGCCGA TTAATGATTA CGGAGCCAAG GTACAGGcgc atgtcatcga 2341 caaatattaa GGATCCtaaC TCGAcgtgca ggcttcctcg ctcactgact cgctgcgctc 2401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatCA ATTCGACCCA 2461 GCTTTCTTGT ACAAAGTTGG CATTATAAAA AATAATTGCT CATCAATTTG TTGCAACGAA 2521 CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT 2581 GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC 2641 TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGCC TCATCTAGAC CAGCCAGGAC 2701 AGAAATGCCT CGACTTCGCT GCTGCCCAAG GTTGCCGGGT GACGCACACC GTGGAAACGG 2761 ATGAAGGCAC GAACCCAGTG GACATAAGCC TGTTCGGTTC GTAAGCTGTA ATGCAAGTAG 2821 CGTATGCGCT CACGCAACTG GTCCAGAACC TTGACCGAAC GCAGCGGTGG TAACGGCGCA 2881 GTGGCGGTTT TCATGGCTTG TTATGACTGT TTTTTTGGGG TACAGTCTAT GCCTCGGGCA 2941 TCCAAGCAGC AAGCGCGTTA CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT 3001 GTTACGCAGC AGGGCAGTCG CCCTAAAACA AAGTTAAACA TCATGAGGGA AGCGGTGATC 3061 GCCGAAGTAT CGACTCAACT ATCAGAGGTA GTTGGCGTCA TCGAGCGCCA TCTCGAACCG 3121 ACGTTGCTGG CCGTACATTT GTACGGCTCC GCAGTGGATG GCGGCCTGAA GCCACACAGT 3181 GATATTGATT TGCTGGTTAC GGTGACCGTA AGGCTTGATG AAACAACGCG GCGAGCTTTG 3241 ATCAACGACC TTTTGGAAAC TTCGGCTTCC CCTGGAGAGA GCGAGATTCT CCGCGCTGTA 3301 GAAGTCACCA TTGTTGTGCA CGACGACATC ATTCCGTGGC GTTATCCAGC TAAGCGCGAA 3361 CTGCAATTTG GAGAATGGCA GCGCAATGAC ATTCTTGCAG GTATCTTCGA GCCAGCCACG 3421 ATCGACATTG ATCTGGCTAT CTTGCTGACA AAAGCAAGAG AACATAGCGT TGCCTTGGTA 3481 GGTCCAGCGG CGGAGGAACT CTTTGATCCG GTTCCTGAAC AGGATCTATT TGAGGCGCTA 3541 AATGAAACCT TAACGCTATG GAACTCGCCG CCCGACTGGG CTGGCGATGA GCGAAATGTA 3601 GTGCTTACGT TGTCCCGCAT TTGGTACAGC GCAGTAACCG GCAAAATCGC GCCGAAGGAT 3661 GTCGCTGCCG ACTGGGCAAT GGAGCGCCTG CCGGCCCAGT ATCAGCCCGT CATACTTGAA 3721 GCTAGACAGG CTTATCTTGG ACAAGAAGAA GATCGCTTGG CCTCGCGCGC AGATCAGTTG 3781 GAAGAATTTG TCCACTACGT GAAAGGCGAG ATCACCAAGG TAGTCGGCAA ATAACCCTCG 3841 AGCCACCCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC 3901 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC 3961 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 4021 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 4081 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 4141 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 4201 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 4261 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT 4321 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 4381 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 4441 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 4501 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG 4561 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTcctagg 4621 TGTAAAACGA CGGCCAGTCT TAAGCTCGGG CCCCAAATAA TGATTTTATT TTGACTGATA 4681 GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT TTATAATGCC AACTTTGTAC 4741 AAAAAAGCAG GCTCCGAATT Ggtatcacga ggcagaattt cagataaaaa aaatccttag 4801 ctttcgctaa ggatgatttc tgGAATTCAT GA //