LOCUS       pCM224       6224 bp    DNA   CIRCULAR SYN        22-SEP-2006
DEFINITION  
ACCESSION   pCM224
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             459..1268
                     /gene="KanR"
                     /product="KanR"
     CDS             complement (1546..2553)
                     /gene="tTA"
                     /product="tTA transactivator"
     misc_feature    complement (2554..3324)
                     /gene="Pcmv"
                     /product="CMV promotor"
     misc_feature    3335..3546
                     /gene="Tadh1"
                     /product="ADH1 transcription terminator"
     misc_feature    3552..3651
                     /gene="TetO"
                     /product="2 copies of TetO box"
     misc_feature    3652..3804
                     /gene="Pcyc1"
                     /product="CYC1 TATA region"
     CDS             complement (4897..5757)
                     /gene="AmpR"
                     /product="AmpR"
BASE COUNT     1589 a   1490 c   1458 g   1687 t
ORIGIN      
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
      121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      421 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
      481 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      541 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
      601 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
      661 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
      721 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
      781 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
      841 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
      901 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
      961 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
     1021 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1081 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1141 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1201 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1261 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     1321 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     1381 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     1441 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
     1501 agtgtcgaaa acgagctcga attcttatta cgatcctcgc gccccctacc caccgtactc
     1561 gtcaattcca agggcatcgg taaacatctg ctcaaactcg aagtcggcca tatccagagc
     1621 gccgtagggg gcggagtcgt ggggggtaaa tcccggaccc ggggaatccc cgtcccccaa
     1681 catgtccaga tcgaaatcgt ctagcgcgtc ggcatgcgcc atcgccacgt cctcgccgtc
     1741 taagtggagc tcgtccccca ggctgacatc ggtcgggggg gccgtcgaca gtctgcgcgt
     1801 gtgtcccgcg gggagaaagg acaggcgcgg agccgccagc cccgcctctt cgggggcgtc
     1861 gtcgtccggg agatcgagca ggccctcgat ggtagacccg taattgtttt tcgtacgcgc
     1921 gcggctgtac gcggacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa
     1981 ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg
     2041 tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg
     2101 atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata
     2161 taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt
     2221 ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact
     2281 tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc
     2341 ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag
     2401 caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg
     2461 ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct
     2521 gttaatcact ttacttttat ctaatctaga catatgaatt aattcgggcc gcggaggctg
     2581 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
     2641 tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc
     2701 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     2761 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     2821 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     2881 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     2941 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     3001 tgatgtactg ccaagtgggc agtttagcgt aaatactcca cccattgacg tcaatggaaa
     3061 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     3121 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     3181 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     3241 caacatggcg gtaatgttgg acatgagcca atataaatgt acatattatg atatggatac
     3301 aacgtatgca atgggccaag ctcctcgagt aattcgcgcc acttctaaat aagcgaattt
     3361 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     3421 ttaaagtgac tcttaggttt taaaacgaaa attcttgttc ttgagtaact ctttcctgta
     3481 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     3541 cagatcaatt cctcgatccc tatcagtgat agagagtcga caaagtcgag tttctcgatc
     3601 cctatcagtg atagagagtc gacaaagtcg agtttctcga tcgagaccac tgcatgcatg
     3661 tgctctgtat gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata
     3721 cattaggtcc tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac
     3781 acactaaatt accggatcaa ttcgggggat ccgtttatca gatccactag tggcctatgc
     3841 ggccgcggat ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa
     3901 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4321 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4561 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4861 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4921 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4981 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5041 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5101 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5161 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5221 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5281 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5341 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5401 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5461 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5521 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5581 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5641 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5701 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5821 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5881 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5941 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6001 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6061 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     6121 gattgtactg agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata
     6181 cataacctta tgtatcatac acatacgatt taggtgacac tata
//