% % create_model_pop_ctrl % % create the reactions for each cell and purely external reactions % % transcription add_reaction({'P_luxR'}, {'P_luxR' 'LuxI' 'LuxR' 'Killer'}, 0.1); add_reaction({'P_luxR_LuxR_AHL'}, {'P_luxR_LuxR_AHL' 'LuxI' 'LuxR' 'Killer'}, 3.0); % promoter regulation add_reaction({'P_luxR' 'LuxR_AHL'}, {'P_luxR_LuxR_AHL'}, 0.1); add_reaction({'P_luxR_LuxR_AHL'}, {'P_luxR' 'LuxR_AHL'}, 0.3); add_reaction({'P_luxR_LuxR_AHL'}, {'P_luxR'}, 0.3); % AHL synthesis, binding, dissociation add_reaction({'LuxI'}, {'LuxI' 'AHL'}, 0.4); add_reaction({'LuxR' 'AHL'}, {'LuxR_AHL'}, 1.0); add_reaction({'LuxR_AHL'}, {'LuxR' 'AHL'}, 0.4); % decay add_reaction({'LuxI'}, {}, 1.0); add_reaction({'LuxR'}, {}, 1.0); add_reaction({'AHL'}, {}, 0.4); add_reaction({'LuxR_AHL'}, {}, 0.4); add_reaction({'Killer'}, {}, 2.0); % % AHL diffusion % % ratio between diffusion in and out of the cell indicates ratio between % a single cell volume and total volume of growth environment % add_reaction({'AHL'}, {'*external*_AHL'}, 100.0); add_reaction({'*external*_AHL'}, {'AHL'}, 1.0); % cell growth add_reaction({'Alive'}, {'Alive' 'Volume'}, 2.0); add_reaction({'create_P_lux'}, {'P_lux'}, 1.0); add_reaction({'create_P_luxR'}, {'P_luxR'}, 1.0); % % add reactions that take place completely outside the cell % add_external_reaction({'*external*_AHL'}, {}, 0.4); % % special instructions for dealing with cell division % add_after_cell_division('create_P_lux', 10); add_after_cell_division('create_P_luxR', 10);