LOCUS pKn003 4797 bp DNA circular 9-JUL-2008 DEFINITION ATCC 37253: very usable general purpose vector. KEYWORDS ATCC. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|487354198| COMMENT VNTDBDATE|487418504| COMMENT LSOWNER| COMMENT VNTNAME|pKn003| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTAUTHOREML|mgethers@mit.edu| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers promoter complement(4632..4632) /vntifkey="30" /label=P(BLA) /note=" bla gene promoter " CDS complement(3740..4597) /vntifkey="4" /label=AP(R) /note=" bla gene- Ap(r) determinant " promoter complement(2618..2618) /vntifkey="30" /label=P(LAC) /note=" lac promoter " rep_origin 2978..2978 /vntifkey="33" /label=ORI /note=" RNaseH cleavage point " misc_feature 2588..2611 /vntifkey="21" /label=M13\Reverse\Primer\Binding\Site misc_feature 352..375 /vntifkey="21" /label=M13\Forward\Primer\Binding\Site misc_feature 1522..2547 /vntifkey="21" /label=HmgA\Downstream\Fragment misc_feature 2528..2547 /vntifkey="21" /label=BT2724 misc_feature 420..437 /vntifkey="21" /label=BT2697 misc_feature 1487..1504 /vntifkey="21" /label=BT1188 misc_feature 420..1504 /vntifkey="21" /label=HmgA\Upstream\Fragment BASE COUNT 1008 a 1485 c 1336 g 968 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 ctcggagact gcgtcatcca ccctgggtgc cgtcataccc catccgccaa ctgatagccc 481 gagtcgtcgc aagcagccgt tccgccgctg gcccctgctc ctcggcctgg aagatgctcg 541 ccgggccgac caccgtgagc accgccgcca cccgcccgcg ggcgtcgaac accggtgccg 601 agagcgcctc caccccaggc atcagcagcc catggatcgc atgcaggccg cgcgcgcgga 661 tgccctccag gagcaccgcg taggccgccg ggtcggccag cggatggtcg gcacgcccgg 721 ccaactcttc ctcgcgcagc tcggccacct cgcgttccgg caggaacgcg gcgaacacca 781 ggccggtgga cgaaccgagc agcggcagca ccgagccgac ctgggtgacc accgtcaccg 841 cgcgcaccgc ctgttccacc tgtaccacgg tggcgccacg gttgccccag acggcgagga 901 agcaggtctc gttgagcacg tcgcgcagct cggccagcgg cgcggcggcg ctcttcagca 961 cgtccatgct gtccagcgcc gccaggccca cgcgcagcgc ctcgcgcccc agcgagtaat 1021 gattggtgga ggcgtcctgg accgcgaagc cgctggcgat caatgcctgc agatagcgat 1081 ggaccttgct cgccggcatc cccacatgct ccgccaggcg cgacagcgag gtcgccgggg 1141 agagttcggc gagggccttg aggatatcgg taccgacttc ggcggagcgg accttctgct 1201 tgccggaggt ttcggcggga ctgctgttct tttccatgga cgcgggttcc tgcgggcggg 1261 gccgacgtta tagcttgacg ctgccttcca ctcaaattac gttatgcgta attgaattac 1321 gataaaaata acgcagccag cgtgccaggt ccccggcgga tcggcggcaa gctgccccca 1381 ctccccggag gcctcagatg aacctcgact ccactgccct cgcctatcaa tcgggcttcg 1441 gcaacgaatt cagcagcgaa gcgctccccg gcgccctgcc ggtcggccag aactccccgc 1501 agaagactct agaggatccc cccatggcct tcatgttcga gaccagccag gtcctccggc 1561 cgagccgtgc cgccctcgag acgccggccc tgcagaatga ctacgatgcc tgctgggcgt 1621 cgctcgtatc caccttcaac ccgcaacgga gataacccca tgaaccagcc aacccccact 1681 cgtagctggg tcgcctccgc caatgggcat gccgactttc ccctgcaaaa cctgccgctg 1741 ggcatcttca gccgtgccgg cctgagcccg cgttgcggcg tggccatcgg cgactcgatc 1801 ctcgacctgg acgcggcgct ggccgccggg ctgttcgagg gtgccgccgg cgaagccgcc 1861 gaagccaccc gcggcggcgc gctgaacgcc ttcttcgccc tcggcaagcc ggcccgcgtg 1921 gcgctgcgcc agcgcctgct ggagctgctc ggcgaaggcg ccgcggagcg cccggaactg 1981 ctccatccgg ccgccgagtg ccaaatgcac ctaccggcca ggatcggcga ctacaccgac 2041 ttctacgtcg gcatccgcca tgccgagaac gtcggcaagc tgttccgccc ggacaaccca 2101 ctgctgccca actacaagca cgtgcccatc ggctaccacg gccgcgcctc gaccgtgcgc 2161 gtctccggca gcgaagtgcg ccggcccaac ggccagaccc tgccggccgg cgccagcgag 2221 ccggtgttcg gcccctgcgc gcgcctggac tacgaactgg agctgggcat ctggatcggc 2281 cagggcaacg ccctcggcga agccatcccg gtttcccgcg ccgccgagca catcgccggc 2341 ttctgcctgc tcaacgactg gtcggcgcgc gacatccagg cctgggaata ccaaccgctc 2401 ggcccgttcc tgtcgaagag cttcattacc agcgtctcgc cctgggtggt caccgccgaa 2461 gccctggagc cgttccgccg tgcccagcca gcgcgccccg aaggcgaccc gcggccgctg 2521 ccgtatctct acgacgacaa cgaccagggg taccgagctc gaattcgtaa tcatggtcat 2581 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 2641 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 2701 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 2761 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 2821 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 2881 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 2941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 3421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 3661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 3721 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 3781 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 3841 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 3901 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 3961 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4021 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4081 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4141 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4201 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 4261 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 4321 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 4381 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 4441 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 4501 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 4561 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 4621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 4681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 4741 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc //