LOCUS New_DNA 3014 bp ds-DNA circular 05-FEB-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 2803..2820 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1076..1096) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2836..2935 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(913..932) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2944..2965 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 940..1039 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1308..2318 /label=specR /ApEinfo_fwdcolor=#f2f09d /ApEinfo_revcolor=#f2f09d /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1056..1075) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(3014..3014,1..5) /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 835..840 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3005..3010 /label=EcoRI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2321..2326 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1168..1173 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(380..385,400..401) /label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(646..651,666..667) /label=AcuI/Eco57I(1) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1650..1655,1670..1671) /label=AcuI/Eco57I(2) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2997..3002,3..4) /label=AcuI/Eco57I(3) /ApEinfo_label=AcuI/Eco57I /ApEinfo_fwdcolor=#d2d068 /ApEinfo_revcolor=#d2d068 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(154..159,168..169) /label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(2324..2329,2338..2339) /label=BseRI(1) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(838..839,848..853)) /label=BseRI(2) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(1154..1155,1164..1169)) /label=BseRI(3) /ApEinfo_label=BseRI /ApEinfo_fwdcolor=#9ac688 /ApEinfo_revcolor=#9ac688 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2328..2796 /label=colE2 Ori /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=#0080c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(join(2738..2741,2743..2748)) /label=BsaI /ApEinfo_fwdcolor=#c9cc9d /ApEinfo_revcolor=#c9cc9d /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 166..831 /label=AmilCP /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6..135 /label=PglpT /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 GATCTgaaag tgaaacgtga tttcatgcgt cattttgaac attttgtaaa tcttatttaa 61 taatgtgtgc ggcaattcac atttaattta tgaatgtttt cttaacatcg cggcaactca 121 agaaacggca ggttcGGATC TGACTAATTT AACGAGGAGG ATTTCatgag tgtgatcgct 181 aaacaaatga cctacaaggt ttatatgtca ggcacggtca atggacacta ctttgaggtc 241 gaaggcgatg gaaaaggtaa gccctacgag ggggagcaga cggtaaagct cactgtcacc 301 aagggcggac ctctgccatt tgcttgggat attttatcac cacagtgtca gtacggaagc 361 ataccattca ccaagtaccc tgaagacatc cctgactatg taaagcagtc attcccggag 421 ggctatacat gggagaggat catgaacttt gaagatggtg cagtgtgtac tgtcagcaat 481 gattccagca tccaaggcaa ctgtttcatc taccatgtca agttctctgg tttgaacttt 541 cctcccaatg gacctgtcat gcagaagaag acacagggct gggaacccaa cactgagcgt 601 ctctttgcac gagatggaat gctgctagga aacaacttta tggctctgaa gttagaagga 661 ggcggtcact atttgtgtga atttaaaact acttacaagg caaagaagcc tgtgaagatg 721 ccagggtatc actatgttga ccgcaaactg gatgtaacca atcacaacaa ggattacact 781 tcggttgagc agtgtgaaat ttccattgca cgcaaacctg tggtcgccta ataaGGATCC 841 taaCTCGCTC CTCaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 901 cgagcggtat cagctcactc aaaggcggta atCAATTCGA CCCAGCTTTC TTGTACAAAG 961 TTGGCATTAT AAAAAATAAT TGCTCATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC 1021 AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG 1081 TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC 1141 AAGATAAAAA TATATCATCA TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT 1201 CGCTGCTGCC CAAGGTTGCC GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC 1261 AGTGGACATA AGCCTGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA 1321 ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG 1381 CTTGTTATGA CTGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC 1441 GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA 1501 GTCGCCCTAA AACAAAGTTA AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC 1561 AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC 1621 ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG 1681 TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG 1741 AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG 1801 TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT 1861 GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG 1921 CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG 1981 AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC 2041 TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC 2101 GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG 2161 CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC 2221 TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT 2281 ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAACC CTCGAGGAGC GCCTCAGCGC 2341 GCCGTAGCGT CGATAAAAAT TACGGGCTGG GGCGAAACTA CCATCTGTTC GAAAAGGTCC 2401 GTAAATGGGC CTACAGAGCG ATTCGTCAGG GCTGGCCTGT ATTCTCACAA TGGCTTGATG 2461 CCGTTATCCA GCGTGTCGAA ATGTACAACG CTTCGCTTCC CGTTCCGCTT TCTCCGGCTG 2521 AATGTCGGGC TATTGGCAAG AGCATTGCGA AATATACACA CAGGAAATTC TCACCAGAGG 2581 GATTTTCCGC TGTACAGGCC GCTCGCGGTC GCAAGGGCGG AACTAAATCT AAGCGCGCAG 2641 CAGTTCCTAC ATCAGCACGT TCGCTGAAAC CGTGGGAGGC ATTAGGCATC AGTCGAGCGA 2701 CGTACTACCG AAAATTAAAA TGTGACCCAG ACCTCGCaaa atgagaccag ataagcctta 2761 tcagataaca gcgccctttt ggcgtctttt tgagcaccta ggTGTAAAAC GACGGCCAGT 2821 CTTAAGCTCG GGCCCCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA 2881 CAAATTGATG AGCAATGCTT TTTTATAATG CCAACTTTGT ACAAAAAAGC AGGCTCCGAA 2941 TTGgtatcac gaggcagaat ttcagataaa aaaaatCCTT AGCTTTCGCT AAGGATCTGA 3001 AGTGgaattc atgA //