LOCUS pNE2001_OleD 5307 bp ds-DNA circular 13-FEB-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature complement(2943..3495) /label=RnaII /ApEinfo_fwdcolor=#0000ff /ApEinfo_revcolor=#0000ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2831..3513 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2074..2733 /label=AmpR /ApEinfo_fwdcolor=#f8c887 /ApEinfo_revcolor=#f8c887 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4085..4963 /label=AraC /ApEinfo_fwdcolor=#83ba01 /ApEinfo_revcolor=#83ba01 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4991..5228 /label=Pbad promoter /ApEinfo_fwdcolor=#017e43 /ApEinfo_revcolor=#017e43 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1439..1596 /label=TrrnB terminator /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1316..1333 /label=6x His Tag /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3595..3614) /label=G00101 /ApEinfo_fwdcolor=#8080ff /ApEinfo_revcolor=#8080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 429..434 /label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 432..437 /label=BB_RBS(1) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 942..947 /label=BB_RBS(2) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1053..1058 /label=BB_RBS(3) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1152..1157 /label=BB_RBS(4) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5295..5300 /label=BB_RBS(5) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(342..347) /label=BB_RBS(6) /ApEinfo_label=BB_RBS /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1259..1264 /label=HindIII /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#9ecfcf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1252..1257 /label=EcoRI /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5228..5233 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3992..3997 /label=SphI /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=#80ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2..1315 /label=OleD /ApEinfo_fwdcolor=#3dfa31 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 CATGGCTACC ACCCAGACCA CTCCCGCCCA CATCGCCATG TTCTCCATCG CCGCCCACGG 61 CCATGTGAAC CCCAGCCTGG AGGTGATCCG TGAACTCGTC GCCCGCGGCC ACCGGGTCAC 121 GTACGCCATT CCGCCCGTCT TCGCCGACAA GGTGGCCGCC ACCGGCGCCC GGCCCGTCCT 181 CTACCACTCC ACCCTGCCCG GCCCCGACGC CGACCCGGAG GCATGGGGAA GCACCCTGCT 241 GGACAACGTC GAACCGTTCC TGAACGACGC GATCCAGGCG CTCCCGCAGC TCGCCGATGC 301 CTACGCCGAC GACATCCCCG ATCTCGTCCT GCACGACATC ACCTCCTACC CGGCCCGCGT 361 CCTGGCCCGC CGCTGGGGCG TCCCGGCGGT CTCCCTCTCC CCGAACCTCG TCGCCTGGAA 421 GGGTTACGAG GAGGAGGTCG CCGAGCCGAT GTGGCGCGAA CCCCGGCAGA CCGAGCGCGG 481 ACGGGCCTAC TACGCCCGGT TCGAGGCATG GCTGAAGGAG AACGGGATCA CCGAGCACCC 541 GGACACGTTC GCCAGTCATC CGCCGCGCTC CCTGGTGCTC ATCCCGAAGG CGCTCCAGCC 601 GCACGCCGAC CGGGTGGACG AAGACGTGTA CACCTTCGTC GGCGCCTGCC AGGGAGACCG 661 CGCCGAGGAA GGCGGCTGGC AGCGGCCCGC CGGCGCGGAG AAGGTCGTCC TGGTGTCGCT 721 CGGCTCGGCG TTCACCAAGC AGCCCGCCTT CTACCGGGAG TGCGTGCGCG CCTTCGGGAA 781 CCTGCCCGGC TGGCACCTCG TCCTCCAGAT CGGCCGGAAG GTGACCCCCG CCGAACTGGG 841 GGAGCTGCCG GACAACGTGG AGGTGCACGA CTGGGTGCCG CAGCTCGCGA TCCTGCGCCA 901 GGCCGATCTG TTCGTCACCC ACGCGGGCGC CGGCGGCAGC CAGGAGGGGC TGGCCACCGC 961 GACGCCCATG ATCGCCGTAC CGCAGGCCGT CGACCAGTTC GGCAACGCCG ACATGCTCCA 1021 AGGGCTCGGC GTCGCCCGGA AGCTGGCGAC CGAGGAGGCC ACCGCCGACC TGCTCCGCGA 1081 GACCGCCCTC GCTCTGGTGG ACGACCCGGA GGTCGCGCGC CGGCTCCGGC GAATCCAGGC 1141 GGAGATGGCC CAGGAGGGCG GCACCCGGCG GGCGGCCGAC CTCATCGAGG CCGAACTGCC 1201 CGCGCGCCAC GAGCGGCAGG AGCCGGTGGG CGACCGACCC AACGGTGGGG GGAATTCGAA 1261 GCTTGGGCCC GAACAAAAAC TCATCTCAGA AGAGGATCTG AATAGCGCCG TCGACCATCA 1321 TCATCATCAT CATTGAGTTT AAACGGTCTC CAGCTTGGCT GTTTTGGCGG ATGAGAGAAG 1381 ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG 1441 CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC 1501 CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA 1561 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT 1621 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG 1681 GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA 1741 GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTTGTTT ATTTTTCTAA 1801 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT 1861 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG 1921 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA 1981 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT 2041 GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT 2101 GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT 2161 TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG 2221 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA 2281 CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT 2341 CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG 2401 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA 2461 CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA 2521 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC 2581 GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT 2641 ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC 2701 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT 2761 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT 2821 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC 2881 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC 2941 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 3001 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 3061 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 3121 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 3181 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 3241 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA 3301 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 3361 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 3421 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 3481 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG 3541 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC 3601 GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG 3661 AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT 3721 TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 3781 TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC 3841 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA 3901 CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC 3961 AGCAGATCAA TTCGCGCGCG AAGGCGAAGC GGCATGCATA ATGTGCCTGT CAAATGGACG 4021 AAGCAGGGAT TCTGCAAACC CTATGCTACT CCGTCAAGCC GTCAATTGTC TGATTCGTTA 4081 CCAATTATGA CAACTTGACG GCTACATCAT TCACTTTTTC TTCACAACCG GCACGGAACT 4141 CGCTCGGGCT GGCCCCGGTG CATTTTTTAA ATACCCGCGA GAAATAGAGT TGATCGTCAA 4201 AACCAACATT GCGACCGACG GTGGCGATAG GCATCCGGGT GGTGCTCAAA AGCAGCTTCG 4261 CCTGGCTGAT ACGTTGGTCC TCGCGCCAGC TTAAGACGCT AATCCCTAAC TGCTGGCGGA 4321 AAAGATGTGA CAGACGCGAC GGCGACAAGC AAACATGCTG TGCGACGCTG GCGATATCAA 4381 AATTGCTGTC TGCCAGGTGA TCGCTGATGT ACTGACAAGC CTCGCGTACC CGATTATCCA 4441 TCGGTGGATG GAGCGACTCG TTAATCGCTT CCATGCGCCG CAGTAACAAT TGCTCAAGCA 4501 GATTTATCGC CAGCAGCTCC GAATAGCGCC CTTCCCCTTG CCCGGCGTTA ATGATTTGCC 4561 CAAACAGGTC GCTGAAATGC GGCTGGTGCG CTTCATCCGG GCGAAAGAAC CCCGTATTGG 4621 CAAATATTGA CGGCCAGTTA AGCCATTCAT GCCAGTAGGC GCGCGGACGA AAGTAAACCC 4681 ACTGGTGATA CCATTCGCGA GCCTCCGGAT GACGACCGTA GTGATGAATC TCTCCTGGCG 4741 GGAACAGCAA AATATCACCC GGTCGGCAAA CAAATTCTCG TCCCTGATTT TTCACCACCC 4801 CCTGACCGCG AATGGTGAGA TTGAGAATAT AACCTTTCAT TCCCAGCGGT CGGTCGATAA 4861 AAAAATCGAG ATAACCGTTG GCCTCAATCG GCGTTAAACC CGCCACCAGA TGGGCATTAA 4921 ACGAGTATCC CGGCAGCAGG GGATCATTTT GCGCTTCAGC CATACTTTTC ATACTCCCGC 4981 CATTCAGAGA AGAAACCAAT TGTCCATATT GCATCAGACA TTGCCGTCAC TGCGTCTTTT 5041 ACTGGCTCTT CTCGCTAACC AAACCGGTAA CCCCGCTTAT TAAAAGCATT CTGTAACAAA 5101 GCGGGACCAA AGCCATGACA AAAACGCGTA ACAAAAGTGT CTATAATCAC GGCAGAAAAG 5161 TCCACATTGA TTATTTGCAC GGCGTCACAC TTTGCTATGC CATAGCATTT TTATCCATAA 5221 GATTAGCGGA TCCTACCTGA CGCTTTTTAT CGCAACTCTC TACTGTTTCT CCATACCCGT 5281 TTTTTTGGGC TAACAGGAGG AATTAAC //