LOCUS pBgl00001_Brp000 2863 bp ds-DNA linear 06-FEB-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 2662..2679 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(200..1059) /label=Beta Lactamase (full length) /ApEinfo_fwdcolor=#fcfa98 /ApEinfo_revcolor=#fcfa98 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1385..1405) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 397..1056 /label=AmpR /ApEinfo_fwdcolor=#f8c887 /ApEinfo_revcolor=#f8c887 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2695..2794 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 175..180 /label=HindIII /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#9ecfcf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1795..2635) /label=p15A origin /ApEinfo_fwdcolor=#956a92 /ApEinfo_revcolor=#956a92 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1144..1144 /label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1549..1785 /label=DHFR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 16..159 /label=Pctx promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1222..1241) /label=G00101 /ApEinfo_fwdcolor=#99e4fb /ApEinfo_revcolor=#99e4fb /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 181..199 /label=RBS hit A2 /ApEinfo_fwdcolor=#792aff /ApEinfo_revcolor=#792aff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2803..2824 /label=CA998 /ApEinfo_fwdcolor=#9bd1f9 /ApEinfo_revcolor=#9bd1f9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1249..1348 /label=attL2 /ApEinfo_fwdcolor=#e9b4fa /ApEinfo_revcolor=#e9b4fa /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1365..1384) /label=T7 Universal /ApEinfo_fwdcolor=#b19943 /ApEinfo_revcolor=#b19943 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10..10 /label=BglII /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1145..1149 /label=BamHI(1) /ApEinfo_label=BamHI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1..6 /label=EcoRI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1786..1791 /label=XhoI /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#00ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1477..1482 /label=XbaI /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gaattcatgA GATCTaacag aaaatgataa aaaaggacta atagtatatt ttgatttttg 61 atttttgatt tttgattttt gatttttgat ttttgatttt tgatttcaaa taatacaaat 121 ttatttactt atttaattgt tttgatcaat tatttttctg GATCTtacga cgggAAGCTT 181 AAATCCTAAT GGTTTATTTt gagtattcaa catttccgtg tcgcccttat tccctttttt 241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 1021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 1081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 1141 cttGGATCCt aaCTCGCTCC TCaggcttcc tcgctcactg actcgctgcg ctcggtcgtt 1201 cggctgcggc gagcggtatc agctcactca aaggcggtaa tCAATTCGAC CCAGCTTTCT 1261 TGTACAAAGT TGGCATTATA AAAAATAATT GCTCATCAAT TTGTTGCAAC GAACAGGTCA 1321 CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT 1381 ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT 1441 ACATTGCACA AGATAAAAAT ATATCATCAT GCCTCCTCTA GAgttgacaa ttaatcatcg 1501 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccAT GGGTCAAAGT 1561 AGCGATGAAG CCAACGCTCC CGTTGCAGGG CAGTTTGCGC TTCCCCTGAG TGCCACCTTT 1621 GGCTTAGGGG ATCGCGTACG CAAGAAATCT GGTGCCGCTT GGCAGGGTCA AGTCGTCGGT 1681 TGGTATTGCA CAAAACTCAC TCCTGAAGGC TATGCGGTCG AGTCCGAATC CCACCCAGGC 1741 TCAGTGCAAA TTTATCCTGT GGCTGCACTT GAACGTGTGG CCTAACTCGA Gtagaaatat 1801 tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc 1861 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac 1921 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt 1981 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc 2041 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa 2101 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc 2161 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga 2221 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg 2281 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt 2341 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc 2401 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc 2461 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat 2521 cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc 2581 acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct 2641 gatgtccggc ggtgccctag gTGTAAAACG ACGGCCAGTC TTAAGCTCGG GCCCCAAATA 2701 ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 2761 TTTATAATGC CAACTTTGTA CAAAAAAGCA GGCTCCGAAT TGgtatcacg aggcagaatt 2821 tcagataaaa aaaatCCTTA GCTTTCGCTA AGGATCTGAA GTG //