LOCUS pPRL_pUC18_BBa_K27 10027 bp ds-DNA linear 03-JAN-2012 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT /Users/nkuldell/Desktop/pUC18.ape from 1 to 2686 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 378..395 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind complement(7802..7822) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin complement(8190..8872) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS complement(239..307) /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature complement(7777..7796) /label=BB prefix (RI, XbaI) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_binding complement(7828..7850) /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed misc_feature complement(411..431) /label=BB suffix (Spe, Pst) /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green CDS complement(8970..9629) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagcggc 421 cgctactagt attagcgctt ggccgcgaaa accgcgtccg ggatctccgc ctcgctcaca 481 ttcggaaaat cacgcaggct tgcacgcgac gcttcatcgc cggtgaccat gcgcagtgga 541 gtgccgctcg ccgcatccag gtacagggtg ctcggacctt tacctgggcg ctggtaacgc 601 caaccgtccg cttcgcgacc cagaaccacg cgacggccaa tgtgacgggc acccagacgg 661 cgcaggacat cgcgtggcag gaacaattcg gcaaaagggc cagtctcatc atccagcgga 721 cgctcacaca gctgttcacc cagggcggta cgctcacgac cataggcgac tttttgtttc 781 caggtacggc cgctggccgc attaccaacc tccgacatcc acaggcgata accagtatca 841 cgctcgctcc acggattgaa caggccgtca atacgacaga tgtcacgttc atagtcgaac 901 cagcaatagc cgctggtcag ctggtcatcc ggcagcatcg ggctccaata agagacatag 961 gcgctgctcc aacgggctgg caacagtggt ggctcacggt tctccatttt ttttacctcc 1021 ttaatgatca tcagcgctgc aaagcataac gcagattctg cgccagtgct tccggcatcg 1081 gcggcaggct tttgcggcgt gcgtcgaagc tttgcacaaa ttccgccgag gacaggtgca 1141 tgcgctcttc gacggtttcg aaccaaacgc ggctgttgtc tgcgtggcca cggaacaact 1201 gcaccagcgg cagggcacgc tcttcgaaac gtttcagcgc ggcaggcaca ccatcttcgg 1261 tacacagcgc tttaaccagc agctgcgcca ccaccacggc catcgtggtg ccgtggccga 1321 tgctaaagtg accgctttgc agcgcgtcac ccagcagaac caacttacca tcatgacaac 1381 ggtcatgaga caacgtcatg aagttacgcc aacccagacc cggctggctc accaggccgt 1441 gaccacccag ctcggcctgg aacaccttag caacgtattc tgcgctcgcc tcttcggaca 1501 tttcgcccag gcgtgcgcgt gcgtaagtct cttcgctaca ttcgacaatg aacgtgctca 1561 tggtatcgct atacttatag gcatgcgcga taaagatatc tttaccatgg gtacgaaaaa 1621 ccagattcat ctgatcgaac agctggctag tgccatacca aatgtactta ttgcggccgt 1681 agtccacctg cgggaccaga gcctcggtga aatgcgcggt tttgtgatta acaccattag 1741 ccaggaccac cagatcatag tccgccagcg gcagctcacc gtgttccagc aacgggcttt 1801 cgaaacgaat agcaatgcct tggctgcggc acttatcgcg cagcgcgtga accagaccgc 1861 gacgctccac gccgcacaac aaaacgcccg tggacatcaa ggacggctca ttatgatgca 1921 ccagtttgaa gtcctccaga aattgcggat tcagacgctc cggtgcatcc agataggaca 1981 gcgggttcgc cgggtgctga cccggacggc caggcagcac gacaccccag cccagcactt 2041 cttgctcgtc attcttctcc acgatgtcaa tggcccacaa agggcgtgcc tgcttcagtt 2101 gggatgcgaa aaccagacca gctggaccag caccaatgac cagaatcttc atttttttta 2161 cctccttaaa gatcttcaat tcacgcgacc aatcttgtac caaacattct gtttacgctg 2221 gatttgttgc agacgatcgt acggctcgct cgtaaagtac agtttctcgg ccatatccgg 2281 cggatacagg cccggaaact tggaatgcat gtaacgcgtg taacgcgcac gcatgaaaaa 2341 gatcgggttc gagcagctca aaacgtcata gttggcgcga gccatgtctt gcattgcgtc 2401 tgcctggact ttgcgcagct ccgtgaactc cggaaaggct ttgtcttggt cgccctggtg 2461 gcggtccagc agctcgacaa acgtgcgggc gtcctccagc gccatgttca taccctgacc 2521 caggaacggc gcagtcgcat gcgcagcatc acccagcaac agcacattac ccttatagtg 2581 aaaggtgcta gagcgcacgt taatcaggtc gttgctcggc ttcgccagaa actgacgcag 2641 catttcgtca cgcgcgtcac gcggcaggcc accgaagtaa cgatcgaaga acgcacgcat 2701 cgtcggttcg tcggtggtcg tcagggaagg gctacccgag tacggcaggc aaacggcgat 2761 gctgacgcta ccatctggga tcgtagccgc acgacccgcg aacaggccac cggaatccat 2821 gccgaaaaag tacagcgtgt ctttacggta acccagtgct tgcgcgtccg gcaaaaccag 2881 ggttttgtag ccatggcgga agaacgtttg ctggaactcg aaacgacgca ggccgctctg 2941 catcgcctga cggacggcgc tgtgggcgcc atccgcaccg ataatcatgt caccttgcag 3001 acgctgcggc tgaccatctt tgccctgaat caacacgctc ttaccatcca ggtcaacatc 3061 caggcactta tgctcaaagt agtaacgaac gcctgccagg ttcgcgtatt tgttcagcag 3121 cttttgaaac gccgcacggt tcaagctcag cggacggaaa tcctccaacg gcttcagttc 3181 gcgcatgcga tactgaccac ccacggagaa agccattgcc acgatcggtt cgccacacgc 3241 atccagctct gcacgcggaa tgcccgcagc caaaacgctc ttgataccac gaacggtcat 3301 gctcacgccg atcgcacggc tgctaacaac gtccacgtaa gagctcagat cgcgcagagg 3361 atcaccacgc ttctccacga cgtgcacttc gtagccacgc ttcgccaggt agatcgcggt 3421 caggccaccc gccaggccgc caccaacgat aatcgcacgt ttcatttttt ttacctcctt 3481 aaggatcctt aggcctcgcg gctcagtttg ccgcacgcca agtccagcat ttggcgacga 3541 tagaaatcca gcagctccat ctgcgcatcc ggcagccaac ccggttccag catgctcgcc 3601 tgctccagca gacgctcgca acggcgtgcc agcatgcgac agcccagcgc gtagtcgtca 3661 tagctacggg tgtcaaccgg acccggcagc ggcggaccgg ccgtgcgacc ggcgatgccg 3721 cttggcaggt tcatcagcgc gcagctcagc ggacgcaaca gaccggtcat cagatcgatt 3781 gctgcgttca tcaggcggct gcgacgcaag ctacccagcg gtttcacggc gaagtgctgc 3841 gccatcatcg caaacatcag ctcataaacg ccttggtaca atgccatcag cgcacgcgca 3901 cgaccgtctg cgacacgttg gcaacccggg ctctcatcca gaaccggatt acgcaacgcc 3961 ggcagcgccg gttcgaacgg tgcgctttga gccatgatac gcgcgctcat ttcacgcaga 4021 cgttgaaaat ggctatgttc gtagtgcggg ctgtccagag cgccaccttc gccctgatcg 4081 gtcacaaatg caataccaaa cagcgccgag tcgcgatcga aaacttccag ttgataaccc 4141 ggatgcgcac ggttggtcag ctcattcagg aacaggtggt gttcaccgcc acgcttacct 4201 gcctcgccac caaacaagtc cggcagatcc aaaaacgcct gacgaatggc ggcatagcag 4261 tccgcgatgg atttgcccgg tgctgggata aagtgcggcc attccagacg aacaaaacgt 4321 gccagcgtgc tttcgctaaa cggttccaga gcgaactcgg tgtccaggcc aaacgcctga 4381 cgtgccgctt cgcccatcag cgggacaccc gcgtagaacg gctcgcccag ggccatcagc 4441 aggttgttaa cgaccaggta atgaatcatt tcttcatgag caatttccag cagtgctgca 4501 cgaataccgc catcacggcg acggtcaccg ctaccgcacg ccagttgcag ctgctcggcg 4561 gtccacgcac cgtcacgaac acgttgttgg ccctgtgcat agttcggaat gctatacgcc 4621 gcgtacaggt attgcagcat cacagacagc tccaggtcga cggctttacg cagctctgcc 4681 gccaactggg ctttagattc aatgcgtgcc ggacccgctg gcggaggtgc ttgcaggcgt 4741 gcctggcctt ccacgtgggc cagatacttc aagaacaaac gagctttcgg tgccgacagt 4801 tcgcgggtgc tcggcatgta ataggacttg ttgcggttct gcggatcaca catctgccac 4861 atcagacgtg cgtacgtttc acatttgcaa cgatcagcca gggaaaacac cttgtcgctc 4921 atgaatggat acaccagctc gtaatacgcc ataacgtggc ggtacaaaaa ggcgtaatcc 4981 acttcttcga cggtcgcgtc atccagcgcc caatcgtcag gcagaacacg cagagggatc 5041 gcctcggcac catcgtccag aacaatgcgt gccggacccg gacgcagacc cgtcagacgc 5101 cattccgcag cgtcgccatc ctgacgagat tcgacgccga ccaggcccat gccctcgata 5161 cgatgcggga tatccggacg cgcacgcgct tcaccgcgaa agtagctgcg cagcgcgatg 5221 ctctcaggga aaaagcgacc gtgacggcga tccggtgcct ccaggtacag gttagacgcg 5281 tcgctttggg tgacccagtc ttgctcacgc cactccgcca gttcgctgct cagggtcaag 5341 ctaccacgcg gttcgcgcag cagcggcagg tccacaatac cgtgattcgt ccaatagtct 5401 tggtacagag cctgcggcac acgtgccaac agtgcgccgt cctcatcacg cagcagcaga 5461 tcgcccagcg gcagtttggc acccaggtcc ggggtcaggc ggtccggtgc gcttggctgc 5521 gcggcacgag tgctgaacgg aatggcacac gccaaattca gcgcaacgcg accaccgttg 5581 acgcgcaggg tcaggtcacc cagacccggt tgacgcggac gcagcagacg accagccggg 5641 tagctcgcca gttcaccacg acgccacaga ccgacaacac cgaccatatc gtgaaaaacc 5701 gggctgttcg gctgaggcgg ggtgctcata ttgaacaacg catattgcac ggtcagaccc 5761 agaacgtcgt catcctccag agccaattgc agacgacgcc aggcctcgga atcaaacgga 5821 cccgggtgaa acagaaaatg tgggtgatct ttcggcacag agaactgaaa caggcgtgcc 5881 agaccaaact cttcatccaa gaagtggccg ccacgctctg caatgtggcc gccacgcgtc 5941 caacgtgcac catggctatc atcaatgtct gccgtaaaca gccacggcgt acccggaccg 6001 gcaccagccg ggctaatggt gaattggccc gcatagattt gtgcagcgtc acggcgcgtc 6061 gggtcgctgt cgacccaacg agcacgattg aaggtggtac gcagataatc attgtagtga 6121 ccccacagtg ccaaacgagc accgaccaga ccgtcaccac gatccgcctc accgccatcc 6181 cattgcacgt ggctaacggt tgcgctctcc cacgaaaagt ggttgttacc ggcagcgttg 6241 tagccctcgg ccaggctgaa cgggccttcc gggtcagcac gaccatccaa gccgaagcgc 6301 ggacccaggg agcgcaggtg acggtggaac tccgtaggat ggcgtgccag gtcgaacggc 6361 tcacccgcca tcgccacggt attgctggcc atatcgatgt ggccgtgcgg atcgcggttc 6421 gcggtcggcg cattgacacg ggcccagcca cggaagtgga tacgcgggaa atccagaatg 6481 ctcatttttt ttacctcctt aatcacgcgg cgatacgctg caacagcaga cggcttgcct 6541 cacgggcgct cagcagaccg ccctccatcc aaccacaatg ctccgtgtac gcatcggagc 6601 acgcgatgat accgctgtcg cgatgagaca gtgcgctcgg gtggtcaata tcagaatcac 6661 ggcaaaactc aacgccgtgc gcccaatact tgtgaacgtg tgccagcggt tgcgggatac 6721 gttcacggac gatacccagt gcgctagcca aatgggtgcg aatttgctcc aggtaaccgt 6781 cctcgccctc cgcgacacaa ccgcgccagt aattcgccat ctcgctatcg gtatagaaga 6841 acaggtactt atcgcctttg aaatagattt tgcgcagcgg gttgtcaaca atcagcacct 6901 gatcgtccag tttgtagtcc aaccaccacg gctcaccgta cgtcagaaag cccttaaaca 6961 gcggcaggct gccatagcgc gcaccggacc aggcttctgg aaaatcaaca ttcaaacccg 7021 ccatcgcgct cggcggaatc gccagaatca gatggcgggt acggtgctcc agtttccagc 7081 cgtcgtcacc tgccagttgc agcaggtagc cgtcaccgtc ggtacggacg ctcagcagac 7141 gataacccag gctaaaacgc gcacctgccg ccttaacctt agccttgatg ccctgaatca 7201 gaccagcaaa gcccgtttcc gctgcaaacc attggttcgc gtcgttgtcc gtcacgctct 7261 ggatctccgg gtgcttaccc acaatgtcgt aggccatttc tgcgctgata tccggcagga 7321 acagtgcgtc gtaacccata gagcggatca taccaaccgc gctatcgtga ccttgataac 7381 ggctgacaaa ctgcaaaaag ctctctttac catgctcttt cagacgcggg gacagttcat 7441 tcatggcgcg cttcagcttt tgctgcacgt gagatttgaa cttcaactgg gtgaacggat 7501 agacttcgct cttttggcta tagtgctgca ttgcgctttg gaaatgcggg tgcaactgag 7561 gggagtagcg acctgcgccc agttcaatgc ttgccttacc atccagcatt ttgctgcgga 7621 tacggccacc ggcttcttgc tgcatgtcaa agatacgcag gctcagacca cggcatgccg 7681 ggctgtccag cagatggctt gcgcacgtca aaccagaaat accagcacca acaatgcaga 7741 tatcggaaga atgtttcatt ttttttacct ccttaactag aagcggccgc gaattcgtaa 7801 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 7861 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 7921 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 7981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 8041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 8101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 8161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 8221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 8281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 8341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 8401 caaagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 8461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 8521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 8581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 8641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 8701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 8761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 8821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 8881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 8941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 9001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 9061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 9121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 9181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 9241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 9301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 9361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 9421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 9481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 9541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 9601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 9661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 9721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 9781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 9841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 9901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 9961 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 10021 tttcgtc //