Num	Seq_name	left_primer	right_primer	left_tm	right_tm	left_gc	right_gc	amplicon_size
1	>GRC13_0019_FC:7:1:2047:12973#AGCT	TTGGATTTCTAGCTGTTGG	CTATGCTGAGGAAAAACGA	53.858	54.035	42.105	42.105	166
2	>GRC13_0019_FC:7:1:7707:17665#AGCT	TCCCTTCCTACACACACAC	CAAAGTTCAAGCACCTTCA	54.116	54.873	52.632	42.105	171
3	>GRC13_0019_FC:7:1:16516:21246#GAAA	CGAACGAAACAATTCAGAG	CAGTGGAAACTAACGAGGA	54.357	53.717	42.105	47.368	148
4	>GRC13_0019_FC:7:2:12046:8170#AGCT	TCCTCTTCTTTCATTCCCT	GAGGAAGCAGGAGAAAAGA	53.814	54.662	42.105	47.368	139
5	>GRC13_0019_FC:7:2:19452:8613#AGCT	GAGCCTTGACATCAACAAG	GGGGTTCAGTAATAATGGC	54.110	54.493	47.368	47.368	147
6	>GRC13_0019_FC:7:2:1707:10004#AGCT	CTAGCCTTGGAAATGTTGA	TGTCTCTCTGTGTGTGCAG	53.858	53.841	42.105	52.632	106
7	>GRC13_0019_FC:7:3:12999:8088#AGCT	AAGGACCAAGATGCTTTCT	GCCTCTCCTACCAAGATGT	53.934	54.195	42.105	52.632	194
8	>GRC13_0019_FC:7:3:1330:18712#AGCT	ATTGCCTAGTTTCCCATTC	AAATGAGGTCAACGAGTGA	54.195	53.922	42.105	42.105	122
9	>GRC13_0019_FC:7:4:1228:3422#AGCT	TTCTACTTGTTTTCCGACG	CAAAGGCTTCGAGTGACTA	53.956	54.080	42.105	47.368	118
10	>GRC13_0019_FC:7:4:7465:6032#AGCT	CCTATGAATTCCTCTTGTCTG	CTAGTGGTGGTTGTGGTTC	54.043	53.693	42.857	52.632	150
11	>GRC13_0019_FC:7:4:12455:15027#AGCT	GTAAGCAACAAGCTGCAAT	TAATGGATTCGTTGAGGAG	54.136	53.578	42.105	42.105	142
12	>GRC13_0019_FC:7:4:15651:17230#AGCT	ACTTTGCACAAAAGGATCA	TGGATGCATTTCTACCATT	54.161	53.878	36.842	36.842	188
13	>GRC13_0019_FC:7:4:17444:17726#AGCT	CGATCTCTACGCATTTCAN	AGAGAGAGGGTAGAGCGG	54.369	54.516	44.444	61.111	102
14	>GRC13_0019_FC:7:5:12296:4780#AGCT	TTCAACAAAAGGGTACCTG	GAAGTTACGGGGCTATTTT	53.603	53.625	42.105	42.105	179
15	>GRC13_0019_FC:7:5:9816:8415#AGCT	GATTGTGTGAGGCAAAGAG	TCCTCACCCTCCCTATATC	54.110	53.892	47.368	52.632	165
16	>GRC13_0019_FC:7:5:11479:16445#GGGA	GGAGTGGGAGAGAGAGAAT	GACCAATCTCATCACCAAA	53.071	54.110	52.632	42.105	165
17	>GRC13_0019_FC:7:5:9715:20268#AGCT	ATTTATGTGCTCGGAAACA	GAAGAGTCCTCCGAGGTAG	54.154	53.895	36.842	57.895	159
18	>GRC13_0019_FC:7:6:10295:5076#AGCT	GTCCATTAGTGAGCAGTGG	TCTCTCAAGGTGTCCTGAA	53.964	54.029	52.632	47.368	131
19	>GRC13_0019_FC:7:6:4873:7763#AGCT	CCGTGAGACTGAGTGAGAG	ACTCCCTTTTCATGGTAGG	54.124	54.086	57.895	47.368	167
20	>GRC13_0019_FC:7:6:15457:8065#AGCT	AAGTTGGTGGAGAGAGAGG	AACGTAAATCTTAACCGCA	54.222	53.565	52.632	36.842	103
21	>GRC13_0019_FC:7:6:13748:13340#CCTT	CTTTGCTCAACATCCACTT	AGGAGTAGAGAGAGAGGCG	53.698	53.796	42.105	57.895	142
22	>GRC13_0019_FC:7:6:3522:16881#AGCT	TTTCTTTCCATGATGACTGA	AGTCTGGCAAAAGTCATTG	54.050	53.698	35.000	42.105	143
23	>GRC13_0019_FC:7:7:15436:12359#AGCT	TAAGGGAGAAAGAGAGGGN	TCTCTACCACAACCACCAC	54.682	54.116	50.000	52.632	123
24	>GRC13_0019_FC:7:7:7125:14431#GAGC	GAGGGAATATTGAAGGAGC	CAGACGATCTTGGTCTTTG	53.798	54.128	47.368	47.368	113
25	>GRC13_0019_FC:7:7:2657:15945#CCAG	CTCTCTCTCTCTTGGTTGCT	CGTTCCAAACAAAGAGTTG	53.867	54.275	50.000	42.105	128
26	>GRC13_0019_FC:7:7:13226:16002#AGCT	TGAACTTACCCACTGGAAG	AGAAGTCGATCGAAGGTG	53.533	53.508	47.368	50.000	157
27	>GRC13_0019_FC:7:8:8188:6461#AGCT	GTGAAGGGTATTTGGTGGT	ATCCAAGCTTCGACTTCTT	54.701	54.108	47.368	42.105	174
28	>GRC13_0019_FC:7:8:12349:10269#AGCT	CACATCTTCAACAAAAGGG	GAAGTTACGGGGCTATTTT	53.998	53.625	42.105	42.105	188
29	>GRC13_0019_FC:7:8:18572:13323#CTTC	TCACTCTCTCCCAAGTTGA	CTGAGTTGAATATCCGTGG	54.029	53.939	47.368	47.368	110
30	>GRC13_0019_FC:7:8:12960:19268#GCGC	GAAAAACCACCGATTGTC	TTAGCCCAAATGCGAGT	53.662	55.188	44.444	47.059	135
31	>GRC13_0019_FC:7:9:18434:2472#TTAT	CCCTCCACACACAAATAAG	ATGGAGAAGAGAGAGCCAT	53.858	53.774	47.368	47.368	167
32	>GRC13_0019_FC:7:10:13213:8272#TCTT	TGTGAAGACTAGAACTGGCA	AAGGTACACACACGAATGC	54.423	54.378	45.000	47.368	148
33	>GRC13_0019_FC:7:10:7077:16334#AGCA	TGAACCAAATTGTATCACAC	TTGAAGGGTGACTTAGTCCT	51.618	53.885	35.000	45.000	176
34	>GRC13_0019_FC:7:10:5690:16861#AGCT	ACCCTAGCATGAACTACCC	GGAGATCAGAAAAACAAAGAA	54.108	53.747	52.632	33.333	167
35	>GRC13_0019_FC:7:10:5998:17074#AGAT	GAGCAGAGGAGTGAGACAA	TTCTCCTCTTCTTCCACNN	53.707	54.050	52.632	47.059	118
36	>GRC13_0019_FC:7:11:9146:9814#AGCT	AAGACGGTGCACGTTATC	CACTCTTAGCCCCTCTCTC	54.452	54.074	50.000	57.895	144
37	>GRC13_0019_FC:7:11:1856:15413#AGCT	AGGATAGGAGGTGAAACCA	TTGCTCGAGTCTGAGAGAG	54.487	54.116	47.368	52.632	136
38	>GRC13_0019_FC:7:12:6576:12689#AGCT	TGCTCAAATAGTTCAAATGC	AGCGGATAGCTACCTCAGT	54.080	54.186	35.000	52.632	169
39	>GRC13_0019_FC:7:12:19049:13078#AGCT	ATGGAGGAAGAACCTGAAC	TGTTGAGTTCGAAAAGCTC	53.975	54.021	47.368	42.105	186
40	>GRC13_0019_FC:7:12:6470:20638#CGCG	AGAGGTTCAGAGCCATTTT	CTGGGCTCTCTCTCTCTCT	53.934	53.689	42.105	57.895	126
41	>GRC13_0019_FC:7:13:15726:1554#AGCT	TGAATCAAAGAACTGGGTG	TTCTTCTCTCTCTCCTCCG	54.415	54.644	42.105	52.632	153
42	>GRC13_0019_FC:7:13:17367:1771#GCTG	GAGCTTTGAGACAGGTGAG	AAAGACAGTCTCTGGGCTC	53.289	54.387	52.632	52.632	127
43	>GRC13_0019_FC:7:13:6778:9176#AGCT	ATTAACGTATGCTCCGAGA	AGAGGAAGAACGAAGAGGA	53.397	54.034	42.105	47.368	115
44	>GRC13_0019_FC:7:13:4326:12776#TGAA	GAATAAATGGCAAATGCAC	GGTTTTTCTTGGATGTGTG	54.091	53.797	36.842	42.105	146
45	>GRC13_0019_FC:7:13:17597:20277#CCTC	GCTCGCTCTCTGTGTGTAT	GAGGTCCTGGTAAACCAAC	53.843	54.389	52.632	52.632	171
46	>GRC13_0019_FC:7:14:7602:2565#AGCT	AGTAAGGGCTTGGTTGATT	TCTCTCCCTCTCCTCCTAA	53.857	53.952	42.105	52.632	141
47	>GRC13_0019_FC:7:14:14796:6477#AGCT	CTCTCGACCAACTCTGAAG	CAGACAGCCTTCTTCTCAA	53.311	53.983	52.632	47.368	144
48	>GRC13_0019_FC:7:14:10084:10233#AGCT	GCCTAAATCGGAACACTAA	TGTTCCACACCTCTCTTTC	52.990	53.392	42.105	47.368	134
49	>GRC13_0019_FC:7:14:5282:11404#TCCA	CCTCTCTCTTCCTCTTTGC	GTGCTCTGAAAGTGGCTAA	54.212	54.062	52.632	47.368	148
50	>GRC13_0019_FC:7:15:6329:2966#AGCT	AACCTCAAAGCTAAGCTCC	TGGTGAAGAATTGAGAGGA	53.896	53.982	47.368	42.105	122
51	>GRC13_0019_FC:7:15:16553:4260#GCGA	GAAGTTACGGGGCTATCTT	TTCAACAAAAGGGTACCTG	53.562	53.603	47.368	42.105	163
52	>GRC13_0019_FC:7:15:15548:7927#TCCC	AAATCAATGTGCTCCTGAA	GTGCGTACGTGTTTGTGTA	54.062	53.910	36.842	47.368	195
53	>GRC13_0019_FC:7:16:10188:15523#AGCT	CTTATAAAGCCTTCTCCCAA	CGGTGAATGGAAAAGTATG	53.953	53.995	40.000	42.105	101
54	>GRC13_0019_FC:7:17:11409:5986#AGCT	GCTTCCTTTTATCCTTTCG	CTCACTCTAGCCTTACCCC	54.291	53.991	42.105	57.895	127
55	>GRC13_0019_FC:7:18:14069:4287#AGCT	ATGGATGAATGTAGGAGCA	TCCATTTCTGTTCTTCCAA	53.817	54.039	42.105	36.842	135
56	>GRC13_0019_FC:7:19:17712:20088#AGCT	AGCTCAACCAAAGGAATTT	ACCTTTGAAGCTTTAGGGA	53.988	54.172	36.842	42.105	155
57	>GRC13_0019_FC:7:20:1194:4138#CCCG	TCTCTCTCTGTCCCTCACA	CCAGCTAGAGAAGGAGAGG	53.968	54.269	52.632	57.895	194
58	>GRC13_0019_FC:7:20:17209:6486#AGCT	AGAGCTCTCCAAAACCAAA	GGTCTTGTGGGTGTACTGA	55.058	54.116	42.105	52.632	169
59	>GRC13_0019_FC:7:21:16352:5089#AGCT	TTCGTCTCTCTGCTCAATC	AAGAGGAAGAAGAGGGTGA	53.859	53.853	47.368	47.368	158
60	>GRC13_0019_FC:7:22:6371:3667#AGCT	TCGTGTTTGGTGTGTAAAA	TGGAGAAGAAGAACACGAG	53.328	53.795	36.842	47.368	144
61	>GRC13_0019_FC:7:22:16923:6401#GCCG	ACGTTGGTCGAGATGTTC	GCGAGTCACAGAGTGAGAG	54.173	54.303	50.000	57.895	158
62	>GRC13_0019_FC:7:22:3339:11596#AGCT	CCTCAACATCACCATCAGT	TAAGGATTTGCAGCAAGAG	54.010	54.210	47.368	42.105	126
63	>GRC13_0019_FC:7:23:3833:4802#ATTA	GCTGAAGCAGCCATTATT	TCCTCTCTCTCAAACTCCC	53.931	54.264	44.444	52.632	144
64	>GRC13_0019_FC:7:23:13788:13917#AGCT	GATTCACGAACACAACTCC	AGAGAGAGAAATGGCTTGG	53.712	54.526	47.368	47.368	166
65	>GRC13_0019_FC:7:23:4327:20090#AGCT	CAGAGTTGAGTTCATGGGA	TGTAGTCCTCCTCCTCCTC	54.371	54.121	47.368	57.895	138
66	>GRC13_0019_FC:7:24:5745:12416#CTGG	CCCTGTTCACACACAGATT	CACACACAGCTCTCCTTTC	54.116	54.160	47.368	52.632	153
67	>GRC13_0019_FC:7:24:14211:12790#AGCT	GAGAAATACGGTGGAGGTT	AACCACCAACAGAACTGAA	54.069	53.838	47.368	42.105	141
68	>GRC13_0019_FC:7:24:13361:17358#AGCT	ACCCACTCCACTCACACTA	TAGTTGGAGCCATTGAANN	53.622	54.217	52.632	41.176	139
69	>GRC13_0019_FC:7:25:8262:7845#AGCT	CTCTACCCTCCAATCATCN	GGAGTGACAAGGGAGAGAT	53.652	53.917	50.000	52.632	134
70	>GRC13_0019_FC:7:25:15783:9167#AGCT	TAGTTGAGGACCTGTGGTG	AACTATTTGAGCTCTCCAGC	54.325	53.906	52.632	45.000	159
71	>GRC13_0019_FC:7:25:12456:20330#CCAC	ATTCCTCCGGACATTTTT	TAACAGAGGCCATTGAGAG	54.406	53.797	38.889	47.368	162
72	>GRC13_0019_FC:7:26:7453:3380#AGCT	AACACTTCTTTCCTGCTCC	TCACACACACACACACACA	54.429	53.870	47.368	47.368	112
73	>GRC13_0019_FC:7:27:6610:2238#GTTT	GTTTTGGAGATTGGGATTT	CAGCCCTCTCTCTCTCTCT	53.987	53.689	36.842	57.895	134
74	>GRC13_0019_FC:7:27:15804:7205#TGAG	ATGGATGATGATTTTGTGG	AAAAACCCAGATCAAAACC	53.919	54.080	36.842	36.842	167
75	>GRC13_0019_FC:7:27:10932:15417#AGCT	GGCAAGCACTGCTATTTAC	AATATTTCTCAGTCGCTTTTT	53.653	52.812	47.368	28.571	187
76	>GRC13_0019_FC:7:28:14232:3093#AGCT	GACCTTGATCTCCCTCATT	TCTCACTCAGCTTCTCAGC	53.874	54.098	47.368	52.632	178
77	>GRC13_0019_FC:7:28:6270:10440#AGCT	GTGTCTCTTCTTAACATCTCCTC	TTTTCTTCCTTCTTCACGN	53.983	54.198	43.478	38.889	144
78	>GRC13_0019_FC:7:29:10214:2994#AGCT	AGAGAAGCGAGAGAATGGT	GCCCATAATACCCAAAACT	54.057	54.112	47.368	42.105	155
79	>GRC13_0019_FC:7:30:2110:15354#TATA	AACACAGCACACAAACACA	AAAAGGCCACATTCTCTCT	54.156	53.934	42.105	42.105	149
80	>GRC13_0019_FC:7:32:18674:15648#GCAG	AAGAGTGTGTGTGGGTTTG	CCATACTGATCTTGCATCC	54.221	53.817	47.368	47.368	131
81	>GRC13_0019_FC:7:34:13043:2226#CCCA	GAGGGAATATTGAAGGAGC	CAGACGATCTTGGTCTTTG	53.798	54.128	47.368	47.368	124
82	>GRC13_0019_FC:7:34:6333:9994#AGCT	CTACCTCGGACATGGTTTT	ACACACACACACACACACAC	55.058	54.002	47.368	50.000	169
83	>GRC13_0019_FC:7:34:4131:16403#AGCT	ATCCTCTCACCATTTTTCC	TATTTGATTCTTGACCCGA	53.932	54.034	42.105	36.842	162
84	>GRC13_0019_FC:7:34:2567:17013#AGCT	TTAATTTGGAAAGCAGTGG	GCAAATTGGACAGCTAAAA	53.915	54.069	36.842	36.842	156
85	>GRC13_0019_FC:7:35:5298:7447#AGCT	ATGGATTGCTTGGAACATA	CCTCATAGTCCGTCCCTAT	53.878	54.029	36.842	52.632	147
86	>GRC13_0019_FC:7:36:17377:2552#GGCA	GTTTTTCTTGGAGATTGGG	TCTCTCTCTCTCTCTCTCTCNN	54.656	53.526	42.105	50.000	177
87	>GRC13_0019_FC:7:37:7292:10400#AGCT	CTCGAATCACCATCAGTTC	ATATATCCTCAAGGGCACC	53.816	53.970	47.368	47.368	168
88	>GRC13_0019_FC:7:37:16601:17976#CACA	ATTTGCCACTAGACCTCCT	AATTCAGAGAACCCCTGAC	53.798	53.975	47.368	47.368	180
89	>GRC13_0019_FC:7:38:10505:13502#AAAA	GCTTCCATTCTGCAGTCTA	CTTAACCACAGTGCACACA	53.962	53.822	47.368	47.368	168
90	>GRC13_0019_FC:7:38:12931:20128#AGCT	AAGCTTTGTGTGTGAGCAT	GAATTTGGAGGGAAAATGT	54.235	53.987	42.105	36.842	156
90	>GRC13_0019_FC:7:39:15699:2869#GCTC
90	>GRC13_0019_FC:7:39:16292:14459#AGCT
90	>GRC13_0019_FC:7:39:19694:20208#AGCT
90	>GRC13_0019_FC:7:39:19676:20221#AGCT
91	>GRC13_0019_FC:7:40:7052:4255#ATCA	GCTAAGCGTGCTAAGAACA	CTAAACGCTCTTCCCTTCT	54.498	53.913	47.368	47.368	164
92	>GRC13_0019_FC:7:40:6269:15095#CGGG	GTGGAAGCCATGGAGTATT	CTGTCTCTCTTTTCCCACC	54.958	54.684	47.368	52.632	161
93	>GRC13_0019_FC:7:41:3221:20097#AGCT	TTAATTTGGAAAGCAGTGG	GCAAATTGGACAGCTAAAA	53.915	54.069	36.842	36.842	156
94	>GRC13_0019_FC:7:42:7070:5132#AGCT	AAACTCCGACGAAACTCA	CATCGTTTTCGTCCTCAT	54.097	54.328	44.444	44.444	141
95	>GRC13_0019_FC:7:42:2021:10823#CGCG	GTGTAGGTGTGGGTGGATA	CACACACATACGAGCGTTA	53.922	54.013	52.632	47.368	145
96	>GRC13_0019_FC:7:42:16176:19683#AGCT	CAAGCTAGAACAACCTCCA	GATAGGGCAGTGAGGGTAG	53.899	54.701	47.368	57.895	199
97	>GRC13_0019_FC:7:43:15416:14979#AGCT	AGATAGGGTGCTGTTTGNN	ATGTGAAATGGCTGAGAGA	54.378	54.006	47.059	42.105	139
98	>GRC13_0019_FC:7:44:16062:21001#AGCT	GAACATCAAGCACATAGGC	TGGCACCTTCACTTATCAT	54.086	53.920	47.368	42.105	134
99	>GRC13_0019_FC:7:45:9551:1682#AGCT	GAACAAGCTGGACATGGT	GGTAACGCTTGACGAAGT	54.173	53.709	50.000	50.000	128
100	>GRC13_0019_FC:7:46:17097:2582#AGCT	CTGTCTCTCTTTTCCCACC	GTACGGGTTAGAGGTTGGT	54.684	54.030	52.632	52.632	121
101	>GRC13_0019_FC:7:46:10667:9294#TCTC	CCGAACTGTCTCAAATACC	GGATGGTGATGAAGATGAA	53.017	54.005	47.368	42.105	171
101	>GRC13_0019_FC:7:47:16088:8382#AGCT
101	>GRC13_0019_FC:7:47:2354:14659#AGCT
102	>GRC13_0019_FC:7:48:15692:5106#AGCT	CACTGTCAGAGAGAGAGAGAGA	TCACACAACAAACACACTCA	53.909	54.143	50.000	40.000	137
103	>GRC13_0019_FC:7:48:15769:7640#AGCT	TGCTCAATGTGTCAAGTCA	TCTCTCTTCTTTCAGACCG	54.313	52.948	42.105	47.368	155
103	>GRC13_0019_FC:7:48:18544:12878#AGCT
104	>GRC13_0019_FC:7:48:1663:14822#AGCT	GGGAGCTGAGGTATTAAGG	AGCCCTCTCTCTATCTCTCTT	53.949	53.270	52.632	47.619	113
105	>GRC13_0019_FC:7:51:6783:6066#AGCT	TCCATAAGTTCAAAAACAACA	CACACTGGAGAAAAGGTTG	53.453	54.044	28.571	47.368	161
106	>GRC13_0019_FC:7:51:17752:17601#AGCT	TCTCTCCAACTACTTCCCC	GAAAGAGAGAGGCAGAAGG	54.172	54.212	52.632	52.632	151
107	>GRC13_0019_FC:7:52:15329:9841#AGCT	CAAGCTAGAACAACCTCCA	GATAGGGCAGTGAGGGTAG	53.899	54.701	47.368	57.895	199
108	>GRC13_0019_FC:7:52:13805:10399#AGCA	CCCTAACTCTTTAATTTCGG	CGTCCCTCTCTTCTTTCTC	53.448	54.034	40.000	52.632	153
109	>GRC13_0019_FC:7:52:5264:12317#AGCT	CAGAAGGATGACCTTGCTA	GCAGAATTGTGGTTTCTTG	53.797	54.161	47.368	42.105	156
110	>GRC13_0019_FC:7:53:1307:8658#CTCA	ATATCGGGTCCCATACTTC	GTTTCCAGCTCCTCCTAAG	53.798	54.125	47.368	52.632	155
111	>GRC13_0019_FC:7:54:7346:14598#AGCT	TGTGTATCGAGATAAGGCG	GGCATTTTCTCTCTCTCNN	54.794	53.710	47.368	47.059	159
112	>GRC13_0019_FC:7:55:7718:2361#AGCT	GAAGCTGTGTTTCAACTGG	TTGACACGAGATTCCATTN	54.211	53.905	47.368	38.889	135
113	>GRC13_0019_FC:7:55:3652:18695#AGCT	AACTCAGCAAGAACACAGC	ACTCTCTCTCTCTGCTGGC	53.905	54.717	47.368	57.895	128
114	>GRC13_0019_FC:7:56:4011:6993#AGCT	ACTGCCATTGAATGATGTT	TGTGGGACCATAGATCAAC	53.717	53.963	36.842	47.368	153
115	>GRC13_0019_FC:7:56:1516:16834#TTGC	AGGGAGAGAATAGAAAGCG	TCTCCTCTCGTTTCTCCA	53.818	54.104	47.368	50.000	168
116	>GRC13_0019_FC:7:57:11922:13486#AGCT	ACCTAAGGGATTAGGGCTT	CAATGAAGAAAAACGGAAA	54.530	53.876	47.368	31.579	195
117	>GRC13_0019_FC:7:57:7418:13938#GGGC	TGGAGTTTGTGCAATAAGG	CCAACAAGAAACGAAAGAA	54.671	53.916	42.105	36.842	160
118	>GRC13_0019_FC:7:58:12436:20463#AGCT	AGCTGAACGAATAGCACTG	CATCTAGAGAACGAGCGTG	54.103	54.537	47.368	52.632	164
119	>GRC13_0019_FC:7:59:19617:20513#AGCT	GGAGGAAAATCAAACCCTA	ATTTGTTGCTGTGTTCGTT	54.173	54.103	42.105	36.842	161
120	>GRC13_0019_FC:7:62:14900:7525#AGCT	TCATCAGAGAGAGAGCAGC	TGACCAAGAATGAAGAAGG	53.991	53.570	52.632	42.105	138
121	>GRC13_0019_FC:7:63:10914:6389#AGCT	GTAAGTGGCCAAGGGTC	GTCTCAATGTCGCCCTC	53.624	54.708	58.824	58.824	136
122	>GRC13_0019_FC:7:63:16354:11569#AGCT	CATAAACATTTTGGCCATT	AGGTAGTGAGAGAAAGTTTGGT	53.559	53.937	31.579	40.909	175
123	>GRC13_0019_FC:7:63:7624:15224#CCTC	CTCTCCTTCTCTAGCTGGC	GGAGAGGAGAGAGACTTTCA	54.429	53.542	57.895	50.000	136
124	>GRC13_0019_FC:7:64:7090:6919#CTCA	CACACAGAAGACCGGTAAC	CTCGGTCTCTCTCTCTCNN	53.881	53.700	52.632	58.824	141
125	>GRC13_0019_FC:7:65:1737:12213#AGCT	TGGATTACAAGCGATCAAT	CATCTCTCTCTGCAGCACT	54.057	53.948	36.842	52.632	193
126	>GRC13_0019_FC:7:65:4735:20276#AGCT	TACCTTCGGAAAAAGAGGT	AAGCATTGGTATTTGTCAGA	54.005	53.291	42.105	35.000	161
127	>GRC13_0019_FC:7:66:2308:1425#AGCT	TTCTTCTTCTTTTACGCGA	TAATTGGGTGAATTGATGG	53.954	54.171	36.842	36.842	137
128	>GRC13_0019_FC:7:68:14654:16468#AGCT	CGAAAGGTTGACTTGAAAA	ATCCCAACTTCCAAATGTT	53.916	54.338	36.842	36.842	115
129	>GRC13_0019_FC:7:70:6243:10993#AGCT	CTCAAATCCTCTCTCTCCC	GAGAGACAAATTTGGAGGG	53.751	54.624	52.632	47.368	155
130	>GRC13_0019_FC:7:71:12095:1295#AGCT	TACTTTTTGCTGAACCAGG	ATGGAGGAATTGGAGAAAC	53.956	53.932	42.105	42.105	118
131	>GRC13_0019_FC:7:71:12088:1315#AGCT	TACTTTTTGCTGAACCAGG	AAGTTGTTCAAGTGCTCCA	53.956	54.211	42.105	42.105	144
132	>GRC13_0019_FC:7:74:16318:2685#CGAG	TCGATCCTCGACAAGGT	GAGGTACGTGAGGTGCTC	54.724	53.871	52.941	61.111	123
133	>GRC13_0019_FC:7:75:10629:13970#CTAG	CAAGAGACTTCTTCGGTCA	CTCTTGCATAGCCAAAGTG	53.795	54.573	47.368	47.368	174
133	>GRC13_0019_FC:7:76:19661:9677#ACGG
134	>GRC13_0019_FC:7:77:13800:15710#CTCA	TCTCTTTCTGTCCCTCACA	CCAGCTAGAGAAGGAGAGG	54.029	54.269	47.368	57.895	178
135	>GRC13_0019_FC:7:78:18787:5835#AGCT	CTCGTTCAACAAGAGCAAC	TTCTCTCGTCTCCCTCTTT	54.395	54.034	47.368	47.368	159
136	>GRC13_0019_FC:7:78:9882:14805#AGCT	TTACTTGGTTGCAGGATTG	CGACACACACAACTGATGA	54.671	54.533	42.105	47.368	116
137	>GRC13_0019_FC:7:78:7594:15141#AGCT	CACATGGTCTAGTTGGCTC	AGAGCCATCAACAAACTCA	53.964	54.110	52.632	42.105	154
138	>GRC13_0019_FC:7:78:2113:15836#AGCT	CTTCTTGCTGAATGCAACT	ATCAAGGATAATAAGCGCC	54.062	54.514	42.105	42.105	130
139	>GRC13_0019_FC:7:78:13493:17218#CAAC	GCTCTCTCTCTCTCTGCCT	TTTCACTTTCCCTGTTGAA	53.857	54.138	57.895	36.842	125
140	>GRC13_0019_FC:7:79:16925:2369#AGCT	TTAGAGATCCTGCTGCTTG	CTCTCAATGGCCTCTGTTA	54.161	53.797	47.368	47.368	150
141	>GRC13_0019_FC:7:79:1496:8310#AGCT	CCTGCATAAGTCCAAACTG	GTGGTCAGGAGAGGAGAGT	54.219	53.962	47.368	57.895	134
142	>GRC13_0019_FC:7:79:1925:11443#AGCT	GGCACTATTACGAAACGG	ACCTTGTCGGTCTGCTC	54.182	54.305	50.000	58.824	160
143	>GRC13_0019_FC:7:80:7383:2958#AGCT	GTTCAAACTTGACACAGCC	AATATCATTGTACGCCAGC	54.007	53.689	47.368	42.105	148
144	>GRC13_0019_FC:7:81:8928:11660#CTCT	GCCTCTCTCTCTGACCAAT	AGAACTCAGCTGGAACCTT	54.289	53.976	52.632	47.368	152
145	>GRC13_0019_FC:7:83:5318:10974#AGCT	AGTTCCTCACTGAAGGCTC	GGCACACAAACCTAGACAG	54.387	54.068	52.632	52.632	154
146	>GRC13_0019_FC:7:83:18406:14374#TAGC	CCCCACTTCTCTGTCTCTC	GAGAAGAGGAAGGAAAGGA	54.651	53.498	57.895	47.368	180
147	>GRC13_0019_FC:7:84:13519:4927#AGCT	TGTGTTTTTGAGGGTCACT	ATACGAACCAATGAAACGA	53.838	53.977	42.105	36.842	142
148	>GRC13_0019_FC:7:84:3449:7179#AGCT	CGAAAACACATGGTAATCC	GGGTGACTCTTGTAGCTGA	53.799	53.629	42.105	52.632	165
149	>GRC13_0019_FC:7:85:4537:18793#AGCT	TCTCTGTCCCTCTCTCTTTC	CAGACAGACAGGCAGGC	53.542	56.152	50.000	64.706	162
150	>GRC13_0019_FC:7:86:15416:11219#CTTG	CTTGCTGTGGGTACGTCT	TCTCTCTGCGAAGCTTTTA	54.527	54.074	55.556	42.105	152
151	>GRC13_0019_FC:7:87:12541:2847#GGGG	AGATTTGGGGTTAGGACAA	TCTCTGCTTCAAAGGCTAA	54.524	53.899	42.105	42.105	178
152	>GRC13_0019_FC:7:87:14292:13581#AGCT	GCGCTTTGTACTTGAGAAG	TCTCTCTTGACCACCACC	53.843	54.092	47.368	55.556	135
153	>GRC13_0019_FC:7:87:13831:18869#AGCT	TGGATTACAAGCGATCAAT	GTCGTGTAGGGAAAGAGNN	54.057	53.883	36.842	52.941	145
154	>GRC13_0019_FC:7:89:17309:8866#ATCC	TCTCTTTCTGTCCCTCACA	CCAGCTAGAGAAGGAGAGG	54.029	54.269	47.368	57.895	155
155	>GRC13_0019_FC:7:90:19228:9330#AGCT	GAGAGAGAGGTAATGGGGA	GGAGGCTCTTCTTTCTGTC	54.074	54.016	52.632	52.632	153
156	>GRC13_0019_FC:7:91:12690:6731#AGCT	TTCAACAAAAGGGTACCTG	GAAGTTACGGGGCTATTTT	53.603	53.625	42.105	42.105	156
157	>GRC13_0019_FC:7:91:7389:10541#AGCT	GAGAGACAGCTATCCACGN	CTAGCGATGTCGTGAGC	54.452	53.530	55.556	58.824	130
158	>GRC13_0019_FC:7:91:13881:12266#GGAC	GGTGTCACTATCTGCTTGG	TCTTTCAGTTGGGATGAGA	53.964	53.982	52.632	42.105	113
159	>GRC13_0019_FC:7:91:7026:21228#CGCA	CGTTGAAAGACCTAACTGC	AGAGAGAGAGAGATGGCGT	53.487	54.002	47.368	52.632	133
160	>GRC13_0019_FC:7:92:9012:9606#AGCT	TTCTGTTTCTTTCTCCGTG	AAGCAAAGAAAGACGATGA	53.858	53.523	42.105	36.842	148
161	>GRC13_0019_FC:7:92:9171:20597#GCTG	AACACAACCAGACAACACC	TCACACACACAGACACACA	54.011	52.838	47.368	47.368	171
162	>GRC13_0019_FC:7:93:12323:19157#AGCT	GAACGCAGAAGAGAGAGTG	CAGTGGTGAGTTCCAACAT	53.482	54.116	52.632	47.368	178
162	>GRC13_0019_FC:7:96:4281:6395#AGCT
163	>GRC13_0019_FC:7:96:4124:14984#AGCT	ATGGATGAATGTAGGAGCA	GCTCCATTTCTGTTCTTCA	53.817	53.736	42.105	42.105	137
163	>GRC13_0019_FC:7:97:5557:1698#AGCT
164	>GRC13_0019_FC:7:97:10060:4565#AGCT	ATTATCGAGGTTAAGCCCA	AGAGGAAGAACGAAGAGGA	54.837	54.034	42.105	47.368	145
165	>GRC13_0019_FC:7:98:8145:13719#GTAG	AACCCTTCAATATCCTTGG	TTGCTCATCATCTCCTAGC	54.042	53.860	42.105	47.368	183
166	>GRC13_0019_FC:7:99:16129:21083#AGCT	AAAGAGGACTACGCTGGTT	CCCTCTCTCTCTCTCTCTCTC	54.069	54.029	47.368	57.143	160
167	>GRC13_0019_FC:7:100:13403:3761#AGCT	GGAGAAGAAAGGAGGAAGA	AGTTCTTCTTTGGGTCCAC	53.498	54.074	47.368	47.368	169
168	>GRC13_0019_FC:7:101:14366:9912#AGAG	GAAAGAAGAGAAGGATGCC	TCAACGGTGAGATTAAGGA	53.972	54.080	47.368	42.105	119
169	>GRC13_0019_FC:7:101:17854:12974#AGCT	CTGCTTGATCGCTTGTAAT	AGAGATGTTGACATCGAGC	54.057	53.498	42.105	47.368	160
170	>GRC13_0019_FC:7:101:6786:15097#AGCT	TCTTCTTTCTCATTTGTAAACATT	ACAAAGTCAAACCCAACAA	54.289	53.899	25.000	36.842	145
171	>GRC13_0019_FC:7:103:2465:6506#AGCT	GAGCCTTGACATCAACAAG	CCTCAGGATCTCTCACAAA	54.110	53.496	47.368	47.368	146
172	>GRC13_0019_FC:7:105:12302:1547#CCCC	CTGTCCCTCTCTCTCTCNN	GGTTTGGTGAACTAGGGTT	53.508	53.991	58.824	47.368	121
173	>GRC13_0019_FC:7:105:17962:6576#AGCT	TCACAACCTAAACGACCAT	TCTCTCTCTCTCTCTGTCTTTTT	53.839	53.587	42.105	39.130	179
174	>GRC13_0019_FC:7:106:12266:1238#AGCT	ATCCATCATAACAAGCCAA	AACAAAGGGTGTGTGTTTG	53.878	54.269	36.842	42.105	155
175	>GRC13_0019_FC:7:106:19825:1941#AGCT	ATGTCTTGCTGAAAACTGC	AAAGAGAGAAAATGGCGTT	53.862	54.156	42.105	36.842	131
176	>GRC13_0019_FC:7:106:4509:11193#AGCT	AGAGAGAGAAGGGTAGCCA	TAGATGATCTTGCTGAGCC	54.074	53.860	52.632	47.368	162
177	>GRC13_0019_FC:7:107:6711:6709#ACAC	CAACACGTAAGACCCTAGC	TGACGGTGTGAAACTAAAAG	53.349	53.890	52.632	40.000	178
178	>GRC13_0019_FC:7:107:15523:8638#ATTT	TGGCTCTCTCTTTGATTTG	CAAATACGAATTGCACACA	53.939	53.925	42.105	36.842	119
179	>GRC13_0019_FC:7:107:15013:14217#AGCT	AGAGATTGCCTTTCCAAAT	CAATTAAAGATCAAGGCCA	53.894	53.819	36.842	36.842	186
180	>GRC13_0019_FC:7:107:5006:15182#AGCT	TAGCAACGACGAGATTCAT	CAGTGATTTCGGTTTTCAC	54.284	53.980	42.105	42.105	139
181	>GRC13_0019_FC:7:108:2791:6719#AGCT	TCGGAAAAACAGTTGGTAG	TTGGTAACTGTCAGACCAAG	53.780	53.649	42.105	45.000	151
182	>GRC13_0019_FC:7:108:17098:10659#CTGA	TGGTGAGTTCATGTTGTTG	CATTCCCTCACTCACAAAG	53.465	53.941	42.105	47.368	187
183	>GRC13_0019_FC:7:108:15355:18442#CCTC	TCTCTTTCTGTCCCTCACA	CCAGCTAGAGAAGGAGAGG	54.029	54.269	47.368	57.895	169
184	>GRC13_0019_FC:7:108:10392:20984#AGCT	TGTGTTTTTGAGGGTCACT	GTGAGCTTCTTCGACTTCA	53.838	53.964	42.105	47.368	120
185	>GRC13_0019_FC:7:109:12910:11929#AGCT	GCTTCCTTTTATCCTTTCG	CCATCTCACTCTAGCCTTNN	54.291	54.231	42.105	50.000	131
186	>GRC13_0019_FC:7:109:6084:16569#AGCA	TGAACCAAATTGTATCACAC	TTGAAGGGTGACTTAGTCCT	51.618	53.885	35.000	45.000	176
187	>GRC13_0019_FC:7:112:12503:14536#GTGA	TCTCTCTGTCTGAGCGTGT	CACTCTCACCAGGAAACAA	54.303	54.472	52.632	47.368	155
188	>GRC13_0019_FC:7:113:16916:11717#AGCT	GTACGAGAGAGCACAGGAG	CACACACACACCAACCC	53.334	54.393	57.895	58.824	100
189	>GRC13_0019_FC:7:114:15345:14906#AGCT	TCCGTGTCACACACACTTA	GCTTCCACACACAAACAGT	54.066	54.393	47.368	47.368	119
190	>GRC13_0019_FC:7:116:7628:8435#CTAC	AACTCCAGTTCACCATCCT	GCAAATCACAATGGGTNN	54.346	54.704	47.368	43.750	129
191	>GRC13_0019_FC:7:117:5605:10726#AGCT	CCTGTTGAAGTCATGCAGT	TGATGACCCAAAAGAGAGA	54.498	53.982	47.368	42.105	174
192	>GRC13_0019_FC:7:118:5565:13061#AGCT	CAATACTGCTAGCGCAAC	CATGGAACAGACCACTCTC	53.006	53.662	50.000	52.632	171
193	>GRC13_0019_FC:7:118:2385:14258#AGCT	AGCAGAGAGAGAAAGGGAG	CCTATTGCTAACATCGCTN	53.755	53.217	52.632	44.444	123
194	>GRC13_0019_FC:7:119:11059:10555#AGCT	TTACGAATGTCTGTGTCCC	AAGTTTGGCCTAAGAAAGC	54.254	53.949	47.368	42.105	184
195	>GRC13_0019_FC:7:120:2578:9134#AGCT	GCCTTGATACTGTCATTGG	TATTAGTGCACATCGCTGA	53.920	53.720	47.368	42.105	139
196	>GRC13_0019_FC:7:120:10499:10625#AGCT	CTCCTCATCATTGAAGCTG	TAACAGAGGCCATTGAGAG	54.212	53.797	47.368	47.368	160
197	>GRC13_0019_FC:7:120:18805:17097#AGCT	AACACAACCAGACAACACC	TCACACACACAGACACACA	54.011	52.838	47.368	47.368	183
198	>GRC13_0019_FC:7:120:6069:18680#AGCT	TCAAAGCTAGAGAGAGCCA	GTCGATGGGTGTTGCTAT	53.839	54.165	47.368	50.000	186