LOCUS AJ852004 4604 bp ds-DNA circular 17-JAN-2009 DEFINITION Synthetic construct pASK88-huHRS3-VH-EP3/1 for humanized anti-CD30 Fab fragment. ACCESSION AJ852004 VERSION AJ852004.1 GI:57337318 KEYWORDS beta lactamase; bla gene; Fab fragment; heavy chain; IGHG1 gene; IGK gene; light chain; tet repressor; tetR gene. SOURCE Cloning vector pASK88-huHRS3-VH-EP3/1 ORGANISM Cloning vector pASK88-huHRS3-VH-EP3/1 other sequences; artificial sequences; vectors. REFERENCE 1 AUTHORS Schlapschy,M. TITLE Humanization of an anti-CD30 Fab Fragment as a reagent for the immunotherapy of Hodgkin's Lymphoma JOURNAL Unpublished REFERENCE 2 (bases 1 to 4604) AUTHORS Schlapschy,M. TITLE Direct Submission JOURNAL Submitted (22-OCT-2004) Schlapschy M., LS Biologische Chemie, TU Muenchen, An der Saatzucht 5, 85354 Freising, GERMANY COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 928..1635 /label=ligh chain precursor /ApEinfo_fwdcolor=#9ef3f8 /ApEinfo_revcolor=#9ef3f8 CDS 147..908 /gene="IGHG1" /note="VH and CH1 domains; mouse-human chimaera; His6 tag for IMAC purification" /codon_start=1 /transl_table=11 /product="Fab fragment heavy chain precursor" /protein_id="CAH65830.1" /db_xref="GI:57337319" /translation="MKKTAIAIAVALAGFATVAQAEVKLQESGGGLVQPGGSLRLSCA ASGFTFTTYTIHWVRQAPGKGLEWVSYINPSSGYSDYNQNFKGRFTTSANKSKNTAYL QMNSLRAEDTAVYYCARRADYGNYKYTWFPYWGQGTLVTVSSASTKGPSVFPLAPSSK STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS SLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH" /label=heavy chain precursor /ApEinfo_fwdcolor=#c5e2e2 /ApEinfo_revcolor=#c5e2e2 terminator 1647..1721 /note="lipoprotein transcription terminator" /label=lipoprotein transcription terminator /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 gene 2310..3170 /gene="bla" /label=bla /ApEinfo_fwdcolor=#f2d08a /ApEinfo_revcolor=#f2d08a gene 3180..3803 /gene="tetR" /label=tetR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 CDS 3180..3803 /gene="tetR" /codon_start=1 /transl_table=11 /product="tet repressor" /protein_id="CAH65833.1" /db_xref="GI:57337322" /db_xref="InterPro:IPR001647" /db_xref="InterPro:IPR003012" /db_xref="InterPro:IPR004111" /db_xref="InterPro:IPR009057" /db_xref="InterPro:IPR011075" /db_xref="InterPro:IPR012287" /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" /label=tet repressor /ApEinfo_fwdcolor=#408080 /ApEinfo_revcolor=#408080 rep_origin 3930..4603 /note="ColEI ori" /direction=RIGHT /label=ColEI ori /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_feature 210..575 /label=VH /ApEinfo_fwdcolor=#dad625 /ApEinfo_revcolor=#dad625 misc_feature 1003..1311 /label=VL /ApEinfo_fwdcolor=#e2cc50 /ApEinfo_revcolor=#e2cc50 ORIGIN 1 acccgacacc atcgaatggc cagatgatta attcctaatt tttgttgaca ctctatcatt 61 gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac 121 aaaaatctag ataacgaggg caaaaaatga aaaagacagc tatcgcgatt gcagtggcac 181 tggctggttt cgctaccgta gcgcaggccg aagttaaact gcaggaatcc ggtggtggtc 241 tggttcagcc aggtggttcc ctgcgtctct cgtgcgctgc ctccggtttc accttcacca 301 cctacaccat ccactgggta cgtcaggccc cgggtaaagg tctggaatgg gtttcctaca 361 tcaacccatc gtccggatac tccgactaca accagaactt caaaggtcgt ttcaccacct 421 cagctaacaa atccaaaaac actgcttacc tccagatgaa ttccctccgt gctgaagaca 481 ctgctgttta ctattgcgcg cgtcgtgccg actacggtaa ctacaaatac acctggttcc 541 cttactgggg tcagggcacg ctggtcaccg tttcctccgc ctccaccaag ggcccatcgg 601 tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc ctgggctgcc 661 tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc gccctgacca 721 gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc ctcagcagcg 781 tggtgactgt gccctccagc agcttgggca cccagaccta catctgcaac gttaatcaca 841 aacccagcaa caccaaggtc gacaagaaag ttgagcccaa atcttgccat caccaccatc 901 accattaata accatggaga aaataaagtg aaacaaagca ctattgcact ggcactctta 961 ccgttactgt ttacccctgt gacaaaagcc gacatcgagc tcacccaatc cccgtcctcc 1021 ctgtccgctt ccgttggcga ccgtgttacc atcacctgca aggcctccca gaatgtaggc 1081 actaacgtag cttggtacca gcagaaaccg ggtaaagctc cgaaagttct gatctattcc 1141 gcttcctatc gatactccgg tgttccatcc cgtttctctg gatccggttc cggcaccgac 1201 ttcaccctga cgatctcctc cctccagccg gaagacttcg ctacctacta ctgccagcag 1261 taccacacct acccgttaac cttcggtcag ggcaccaaac tcgagatcaa acggactgtg 1321 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgcc 1381 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg 1441 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac 1501 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa 1561 gtctacgcct gcgaagtcac ccatcagggc ctgagttcgc ccgtcacaaa gagcttcaac 1621 cgcggagagt gttaataagc ttgacctgtg aagtgaaaaa tggcgcacat tgtgcgacat 1681 tttttttgtc tgccgtttac cgctactgcg tcacggatct ccacgcgccc tgtagcggcg 1741 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 1801 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 1861 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 1921 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 1981 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 2041 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 2101 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 2161 tattaacgct tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2281 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 2341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 2401 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 2461 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 2521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 2581 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 2641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 2701 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 2761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 2821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 2881 tattaactgg cgaactactt actctagctt cccggcaaca attgatagac tggatggagg 2941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 3001 ataaatctgg agccggtgag cgtggctctc gcggtatcat tgcagcactg gggccagatg 3061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 3121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtag gaattaatga 3181 tgtctcgttt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg 3241 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat 3301 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag 3361 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta 3421 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt 3481 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat 3541 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgcagtg gggcatttta 3601 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac 3661 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag 3721 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac 3781 ttaaatgtga aagtgggtct taaaagcagc ataacctttt tccgtgatgg taacttcact 3841 agtttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 3901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4081 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catg //