LOCUS pSH62 7051 bp DNA circular SYN 9-NOV-2005 DEFINITION Recombinase expression vector pSH62, complete sequence. ACCESSION AF298785 VERSION AF298785.1 GI:11344881 SOURCE recombinase expression vector pSH62. ORGANISM recombinase expression vector pSH62 artificial sequences; vectors. REFERENCE 1 (bases 1 to 7051) AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H. TITLE A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast JOURNAL Nucleic Acids Res. 30 (6), E23 (2002) MEDLINE 21882009 REFERENCE 2 (bases 1 to 7051) AUTHORS Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H. TITLE Direct Submission JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie, Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 40225, Germany COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|390669443| COMMENT LSOWNER| COMMENT VNTNAME|pSH62| COMMENT VNTAUTHORNAME|MINGTANG CHEN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast COMMENT VNTUDF|Freezer Position|1|123:51| FEATURES Location/Qualifiers source 1..7051 /organism="recombinase expression vector pSH62" /mol_type="other DNA" /db_xref="taxon:142965" /vntifkey="98" /note="derived from Saccharomyces cerevisiae" gene 504..1163 /gene="HIS3" /vntifkey="60" CDS 504..1163 /gene="HIS3" /codon_start=1 /transl_table=11 /product="imidazoleglycerolphosphate dehydratase" /protein_id="AAG34523.1" /db_xref="GI:11344882" /vntifkey="4" /label=HIS3 terminator 2076..2334 /vntifkey="43" /label=Terminator_1 /note="from CYC1" gene complement(2652..3683) /gene="Cre" /vntifkey="60" CDS complement(2652..3683) /gene="Cre" /codon_start=1 /transl_table=11 /product="recombinase" /protein_id="AAG34524.1" /db_xref="GI:11344883" /vntifkey="4" /label=Cre /note="recombinase" promoter complement(3792..4259) /vntifkey="30" /label=Promoter_P_1 /note="from GAL1" rep_origin 4702..4774 /vntifkey="33" /label=Rep_Origin_1 gene complement(5477..6337) /gene="AmpR" /vntifkey="60" CDS complement(5477..6337) /gene="AmpR" /codon_start=1 /transl_table=11 /product="ampicillin resistance protein" /protein_id="AAG34525.1" /db_xref="GI:11344884" /vntifkey="4" /label=AmpR misc_feature 6476..6990 /vntifkey="21" /label=Misc_Feature_1 /note="CEN6/ARSH4" BASE COUNT 1908 a 1625 c 1611 g 1906 t 1 others ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt 2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg 2161 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac 2221 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag 2281 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg 2341 tcgacaattc caaccttacc caagagttcg ccaaactcag acatcacttt angcaaaacc 2401 gcgccgtgct tcttcctcgg tggcattcat cacgaaatgt tcagcactac gcatactttt 2461 gacaggaaac gcaacggata ttgagtcaat atcaggcatt ctatcgctca gctttacagt 2521 gacaatgacg gctggcgact gaatattagt gcttacagac agcactacat attttccgtc 2581 gatgttgaaa tcctttctca tatgtcacca taaatatcaa ataattatag caatcattta 2641 cgcgttaatg gctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc 2701 agggtacgga tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc 2761 tccggtattg aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt 2821 ccagaccagg ccaggtatct ctgaccagag tcatccttag cgccgtaaat caatcgatga 2881 gttgcttcaa aaatcccttc cagggcgcga gttgatagct ggctggtggc agatggcgcg 2941 gcaacaccat tttttctgac ccggcaaaac aggtagttat tcggatcatc agctacacca 3001 gagacggaaa tccatcgctc gaccagttta gttaccccca ggctaagtgc cttctctaca 3061 cctgcggtgc taaccagcgt tttcgttctg ccaatatgga ttaacattct cccaccgtca 3121 gtacgtgaga tatctttaac cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta 3181 taagcaatcc ccagaaatgc cagattacgt atatcctggc agcgatcgct attttccatg 3241 agtgaacgaa cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca 3301 ccggcatcaa cgttttcttt tcggatccgc cgcataacca gtgaaacagc attgctgtca 3361 cttggtcgtg gcagcccgga ccgacgatga agcatgttta gctggcccaa atgttgctgg 3421 atagttttta ctgccagacc gcgcgcctga agatatagaa gataatcgcg aacatcttca 3481 ggttctgcgg gaaaccattt ccggttattc aacttgcacc atgccgccca cgaccggcaa 3541 acggacagaa gcattttcca ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc 3601 atcaggttct tgcgaacctc atcactcgtt gcatcgaccg gtaatgcagg caaattttgg 3661 tgtacggtca gtaaattgga catttaacac tcagataatg gttttaagta aagtgtacag 3721 gatcggctct gcccctcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg 3781 gatccactag ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat 3841 taacaatttt ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat 3901 gttgaaagta ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat 3961 caaaaatcat cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat 4021 tatcatccta tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact 4081 gccaattttt cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag 4141 cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac 4201 ggaggagagt cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtactaga 4261 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat 4321 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa 4381 gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc 4441 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 4501 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 4561 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4621 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4681 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4741 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4801 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4861 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 4921 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4981 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5041 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5101 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 5161 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5221 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 5281 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 5341 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 5401 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 5461 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5521 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5581 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 5641 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5701 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5761 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 5821 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 5881 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 5941 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 6001 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 6061 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 6121 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 6181 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 6241 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 6301 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 6361 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 6421 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat 6481 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat 6541 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac 6601 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta 6661 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta 6721 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa 6781 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct 6841 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa 6901 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg 6961 tgtaagttac aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat 7021 aaaaataggc gtatcacgag gccctttcgt c //